caa a22 4500 605459916 CHVBK 20210128100236.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00167-014-2923-7 doi (NATIONALLICENCE)springer-10.1007/s00167-014-2923-7 Rotator cuff repair using a decellularized tendon slices graft: an in vivo study in a rabbit model [Elektronische Daten] [Juan Pan, Guo-Ming Liu, Liang-Ju Ning, Yi Zhang, Jing-Cong Luo, Fu-Guo Huang, Ting-Wu Qin] Purpose: Although varieties of surgical repair techniques and materials have been used to repair rotator cuff defects, re-tearing frequently occurs. The purpose of this study is to evaluate the postoperative outcomes of rotator cuff repairs with a decellularized tendon slices (DTSs) graft in a rabbit model. Methods: Large defects in the infraspinatus tendons were created bilaterally in 21 rabbits. The graft group underwent reconstruction of the defects with the DTSs grafts, while the defect group did not undergo any treatment. The specimens underwent histological observation, biomechanical testing, and magnetic resonance imaging (MRI) detection at 4, 8, and 12weeks after surgery. In addition, 2 rabbits that were not operated on were used for MRI detection as a normal reference. Results: Histological analysis revealed that the graft promoted host cell ingrowth and tissue integration, and a tendon-like structure developed at 12weeks. The ultimate tensile load had a significant difference between specimens at 4 and 12weeks in the graft group, but there was no significant difference between the graft group and the defect group. In the graft group, the stiffness at 12weeks was significantly greater than that at 4 or 8weeks, and it was also greater than the stiffness in the defect group at 12weeks. MRI demonstrated that the signal strength of the regenerative tissue from the graft group at 12weeks was similar to that of normal infraspinatus tendon. Conclusion: The DTSs graft allowed for incorporation of host tendon and improved the biomechanical performance of the regenerative tendon. Therefore, the graft could be a promising bioscaffold to enhance the surgical repair of large rotator cuff defects and consequently improve the clinical outcome of rotator cuff tears. Springer-Verlag Berlin Heidelberg, 2014 Rotator cuff tears nationallicence Decellularized tendon slices nationallicence Graft nationallicence Repair nationallicence In vivo nationallicence Rabbit nationallicence Pan Juan Department of Rehabilitation Medicine, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Liu Guo-Ming Department of Orthopaedic Surgery, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Ning Liang-Ju Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Zhang Yi Regenerative Medicine Research Center, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Luo Jing-Cong Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Huang Fu-Guo Department of Orthopaedic Surgery, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Qin Ting-Wu Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1524-1535 0942-2056 23:5<1524 2015 23 167 https://doi.org/10.1007/s00167-014-2923-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-014-2923-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pan Juan Department of Rehabilitation Medicine, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Liu Guo-Ming Department of Orthopaedic Surgery, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Ning Liang-Ju Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yi Regenerative Medicine Research Center, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Luo Jing-Cong Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Huang Fu-Guo Department of Orthopaedic Surgery, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Qin Ting-Wu Institute of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1524-1535 0942-2056 23:5<1524 2015 23 167