caa a22 4500 605459975 CHVBK 20210128100236.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00167-013-2773-8 doi (NATIONALLICENCE)springer-10.1007/s00167-013-2773-8 Distinctive collagen maturation process in fibroblasts derived from rabbit anterior cruciate ligament, medial collateral ligament, and patellar tendon in vitro [Elektronische Daten] [Soki Kato, Mitsuru Saito, Hiroki Funasaki, Keishi Marumo] Purpose: Differences in the tissue-specific collagen maturation process between tendon and ligament are still unknown. Collagen cross-link formation is crucial for the collagen maturation process. The aim of this study is to examine collagen maturation processes of anterior cruciate ligament (ACL), medial collateral ligament (MCL), and patellar tendon (PT) in vitro, in order to determine the optimal cell source for tissue engineering of ligament. Methods: Cells derived from the ACL, MCL, and PT of New Zealand white rabbits were isolated. Each cell type was cultured for up to 4weeks after reaching confluence. Cell-matrix layers were evaluated and compared for their morphology, collagen cross-links, and gene expression levels of lysine hydroxylase 1 and 2, lysyl oxidase (LOX), tenomodulin, collagen1A1 (Col1A1), and collagen3A1 (Col3A1). Results: Transmission electron microscopy photomicrographs verified that collagen fibrils were secreted from all three types of fibroblasts. A higher ratio of dihydroxylysinonorleucine/hydroxylysinonorleucine was evident in the ligament compared to the tendon, which was consistent with lysine hydroxylase 2/lysine hydroxylase 1 gene expression. The gene expression of LOX, which regulates the total amount of enzymatic cross-linking, and the gene expression levels of Col1A1 and Col3A1 were higher in the ACL matrix than in the MCL and PT matrices. Conclusion: ACL, MCL, and PT cells have distinct collagen maturation processes at the cellular level. In addition, the collagen maturation of ACL cells is not necessarily inferior to that of MCL and PT cells in that all three cell types have a good ability to synthesize collagen and induce collagen maturation. This bioactivity of ACL cells in terms of ligament-specific mature collagen induction can be applied to tissue-engineered ACL reconstruction or remnant preserving procedure with ACL reconstruction. The Author(s), 2013 Cell culture nationallicence Collagen nationallicence Cross-linking nationallicence Fibroblast nationallicence Ligament nationallicence Tendon nationallicence Kato Soki Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut Saito Mitsuru Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut Funasaki Hiroki Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut Marumo Keishi Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1384-1392 0942-2056 23:5<1384 2015 23 167 https://doi.org/10.1007/s00167-013-2773-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-013-2773-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato Soki Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Saito Mitsuru Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Funasaki Hiroki Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Marumo Keishi Department of Orthopaedic Surgery, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, 105-8461, Minato-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/5(2015-05-01), 1384-1392 0942-2056 23:5<1384 2015 23 167