caa a22 4500 605460108 CHVBK 20210128100237.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00167-013-2395-1 doi (NATIONALLICENCE)springer-10.1007/s00167-013-2395-1 Effect of a single subacromial prednisolone injection in acute rotator cuff tears in a rat model [Elektronische Daten] [Hyo-Jin Lee, Yang-Soo Kim, Ji-Hoon Ok, Yun-Kyoung Lee, Michelle Ha] Purpose: This study examined the early effect of a subacromial steroid injection on injured rotator cuff tendon. Methods: Forty rats were allocated into two groups: a steroid injection (group 1) and no injection as control (group 2). A full-thickness defect was made at the unilateral infraspinatus tendon in both groups. A single dose of methylprednisolone was injected in steroid group. The tendon was harvested at 1, 3, 7, 14, and 42days after surgery; gene expression and immunohistochemical study were performed for type-I/III collagen, tumour necrosis factor (TNF)-α, and extracellular matrix molecules. Results: The type-III to type-I collagen ratio was at 7days higher in the steroid group than that in the control group and decreased to the control level at 14days and was maintained until 42days. The general expression of the MMPs and TIMPs between two groups showed similar pattern regardless of the steroid injection. The gene expression of aggrecan and fibronectin in the steroid group was significantly higher than that in the control group (p<0.05) at 3days after surgery. They decreased to the equal level with control group at 7days after surgery. Both groups showed no significant difference between aggrecan and fibronectin until 42days after surgery (n.s.). Conclusions: A subacromial steroid injection may alter the collagen composition and extracellular matrix and interfere with the healing process in an acute tear of rat infraspinatus tendon at the early phase after the injection. However, these alterations seem to become normalized after the early inflammatory healing phase. Springer-Verlag Berlin Heidelberg, 2013 Shoulder nationallicence Rotator cuff tear nationallicence Steroid nationallicence Cytokine nationallicence TNF-α nationallicence Lee Hyo-Jin Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut Kim Yang-Soo Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut Ok Ji-Hoon Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut Lee Yun-Kyoung Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut Ha Michelle College of Letters & Sciences, University of California, Berkeley, USA aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/2(2015-02-01), 555-561 0942-2056 23:2<555 2015 23 167 https://doi.org/10.1007/s00167-013-2395-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-013-2395-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Hyo-Jin Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Yang-Soo Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut NATIONALLICENCE 700 1- Ok Ji-Hoon Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Yun-Kyoung Department of Orthopedic Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea, 505 Banpo-dong Seocho-gu, 137-701, Seoul, Korea aut NATIONALLICENCE 700 1- Ha Michelle College of Letters & Sciences, University of California, Berkeley, USA aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/2(2015-02-01), 555-561 0942-2056 23:2<555 2015 23 167