In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field
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| 024 | 7 | 0 | |a 10.1007/s00167-014-3143-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00167-014-3143-x | ||
| 245 | 0 | 0 | |a In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field |h [Elektronische Daten] |c [L. de Girolamo, M. Viganò, E. Galliera, D. Stanco, S. Setti, M. Marazzi, G. Thiebat, M. Corsi Romanelli, V. Sansone] |
| 520 | 3 | |a Purpose: Chronic tendinopathy is a degenerative process causing pain and disability. Current treatments include biophysical therapies, such as pulsed electromagnetic fields (PEMF). The aim of this study was to compare, for the first time, the functional in vitro response of human tendon cells to different dosages of PEMF, varying in field intensity and duration and number of exposures. Methods: Tendon cells, isolated from human semitendinosus and gracilis tendons (hTCs; n=6), were exposed to different PEMF treatments (1.5 or 3mT for 8 or 12h, single or repeated treatments). Scleraxis (SCX), COL1A1, COL3A1 and vascular endothelial growth factor-A (VEGF-A) expression and cytokine production were assessed. Results: None of the different dosages provoked apoptotic events. Proliferation of hTCs was enhanced by all treatments, whereas only 3mT-PEMF treatment increased cell viability. However, the single 1.5mT-PEMF treatment elicited the highest up-regulation of SCX, VEGF-A and COL1A1 expression, and it significantly reduced COL3A1 expression with respect to untreated cells. The treated hTCs showed a significantly higher release of IL-1β, IL-6, IL-10 and TGF-β. Interestingly, the repeated 1.5mT-PEMF significantly further increased IL-10 production. Conclusions: 1.5mT-PEMF treatment was able to give the best results in in vitro healthy human tendon cell culture. Although the clinical relevance is not direct, this investigation should be considered an attempt to clarify the effect of different PEMF protocols on tendon cells, in particular focusing on the potential applicability of this cell source for regenerative medicine purpose, both in surgical and in conservative treatment for tendon disorders. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Pulsed electromagnetic field |2 nationallicence | |
| 690 | 7 | |a PEMF dosage |2 nationallicence | |
| 690 | 7 | |a Human tendon cells |2 nationallicence | |
| 690 | 7 | |a Tendon markers |2 nationallicence | |
| 690 | 7 | |a Anti-inflammatory action |2 nationallicence | |
| 690 | 7 | |a PEMF : Pulsed electromagnetic fields |2 nationallicence | |
| 690 | 7 | |a hTCs : Human tendon cells |2 nationallicence | |
| 700 | 1 | |a de Girolamo |D L. |u Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy |4 aut | |
| 700 | 1 | |a Viganò |D M. |u Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy |4 aut | |
| 700 | 1 | |a Galliera |D E. |u Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, Università degli Studi di Milano, Milan, Italy |4 aut | |
| 700 | 1 | |a Stanco |D D. |u Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy |4 aut | |
| 700 | 1 | |a Setti |D S. |u IGEA SpA, Clinical Biophysics, Carpi, Italy |4 aut | |
| 700 | 1 | |a Marazzi |D M. |u Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy |4 aut | |
| 700 | 1 | |a Thiebat |D G. |u Sport Traumatology Unit, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy |4 aut | |
| 700 | 1 | |a Corsi Romanelli |D M. |u Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy |4 aut | |
| 700 | 1 | |a Sansone |D V. |u Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy |4 aut | |
| 773 | 0 | |t Knee Surgery, Sports Traumatology, Arthroscopy |d Springer Berlin Heidelberg |g 23/11(2015-11-01), 3443-3453 |x 0942-2056 |q 23:11<3443 |1 2015 |2 23 |o 167 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00167-014-3143-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00167-014-3143-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a de Girolamo |D L. |u Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Viganò |D M. |u Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Galliera |D E. |u Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, Università degli Studi di Milano, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stanco |D D. |u Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Setti |D S. |u IGEA SpA, Clinical Biophysics, Carpi, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marazzi |D M. |u Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Thiebat |D G. |u Sport Traumatology Unit, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Corsi Romanelli |D M. |u Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sansone |D V. |u Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Knee Surgery, Sports Traumatology, Arthroscopy |d Springer Berlin Heidelberg |g 23/11(2015-11-01), 3443-3453 |x 0942-2056 |q 23:11<3443 |1 2015 |2 23 |o 167 | ||