caa a22 4500 605460868 CHVBK 20210128100240.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00167-014-3143-x doi (NATIONALLICENCE)springer-10.1007/s00167-014-3143-x In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field [Elektronische Daten] [L. de Girolamo, M. Viganò, E. Galliera, D. Stanco, S. Setti, M. Marazzi, G. Thiebat, M. Corsi Romanelli, V. Sansone] Purpose: Chronic tendinopathy is a degenerative process causing pain and disability. Current treatments include biophysical therapies, such as pulsed electromagnetic fields (PEMF). The aim of this study was to compare, for the first time, the functional in vitro response of human tendon cells to different dosages of PEMF, varying in field intensity and duration and number of exposures. Methods: Tendon cells, isolated from human semitendinosus and gracilis tendons (hTCs; n=6), were exposed to different PEMF treatments (1.5 or 3mT for 8 or 12h, single or repeated treatments). Scleraxis (SCX), COL1A1, COL3A1 and vascular endothelial growth factor-A (VEGF-A) expression and cytokine production were assessed. Results: None of the different dosages provoked apoptotic events. Proliferation of hTCs was enhanced by all treatments, whereas only 3mT-PEMF treatment increased cell viability. However, the single 1.5mT-PEMF treatment elicited the highest up-regulation of SCX, VEGF-A and COL1A1 expression, and it significantly reduced COL3A1 expression with respect to untreated cells. The treated hTCs showed a significantly higher release of IL-1β, IL-6, IL-10 and TGF-β. Interestingly, the repeated 1.5mT-PEMF significantly further increased IL-10 production. Conclusions: 1.5mT-PEMF treatment was able to give the best results in in vitro healthy human tendon cell culture. Although the clinical relevance is not direct, this investigation should be considered an attempt to clarify the effect of different PEMF protocols on tendon cells, in particular focusing on the potential applicability of this cell source for regenerative medicine purpose, both in surgical and in conservative treatment for tendon disorders. Springer-Verlag Berlin Heidelberg, 2014 Pulsed electromagnetic field nationallicence PEMF dosage nationallicence Human tendon cells nationallicence Tendon markers nationallicence Anti-inflammatory action nationallicence PEMF : Pulsed electromagnetic fields nationallicence hTCs : Human tendon cells nationallicence de Girolamo L. Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy aut Viganò M. Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy aut Galliera E. Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, Università degli Studi di Milano, Milan, Italy aut Stanco D. Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy aut Setti S. IGEA SpA, Clinical Biophysics, Carpi, Italy aut Marazzi M. Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy aut Thiebat G. Sport Traumatology Unit, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy aut Corsi Romanelli M. Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy aut Sansone V. Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy aut Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/11(2015-11-01), 3443-3453 0942-2056 23:11<3443 2015 23 167 https://doi.org/10.1007/s00167-014-3143-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00167-014-3143-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Girolamo L. Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Viganò M. Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Galliera E. Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 700 1- Stanco D. Orthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Setti S. IGEA SpA, Clinical Biophysics, Carpi, Italy aut NATIONALLICENCE 700 1- Marazzi M. Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 700 1- Thiebat G. Sport Traumatology Unit, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy aut NATIONALLICENCE 700 1- Corsi Romanelli M. Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 700 1- Sansone V. Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy aut NATIONALLICENCE 773 0- Knee Surgery, Sports Traumatology, Arthroscopy Springer Berlin Heidelberg 23/11(2015-11-01), 3443-3453 0942-2056 23:11<3443 2015 23 167