Hindlimb-unloading suppresses B cell population in the bone marrow and peripheral circulation associated with OPN expression in circulating blood cells
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| 024 | 7 | 0 | |a 10.1007/s00774-014-0568-8 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00774-014-0568-8 | ||
| 245 | 0 | 0 | |a Hindlimb-unloading suppresses B cell population in the bone marrow and peripheral circulation associated with OPN expression in circulating blood cells |h [Elektronische Daten] |c [Yoichi Ezura, Junji Nagata, Masashi Nagao, Hiroaki Hemmi, Tadayoshi Hayata, Susan Rittling, David Denhardt, Masaki Noda] |
| 520 | 3 | |a Rodent hindlimb unloading (HU) by tail-suspension is a model to investigate disuse-induced bone loss in vivo. Previously, we have shown that osteopontin (OPN, also known as Spp1) is required for unloading-induced bone loss. However, how unloading affects OPN expression in the body is not fully understood. Here, we examined OPN expression in peripheral blood of mice subjected to HU. Real-time RT-PCR analysis indicated that OPN expression is increased in circulating peripheral blood cells. This HU-induced increase in OPN mRNA expression was specific in circulating peripheral blood cells, as OPN was not increased in the blood cells in bone marrow. HU-induced enhancement in OPN expression in peripheral blood cells was associated with an increase in the fraction of monocyte/macrophage lineage cells in the peripheral blood. In contrast, HU decreased the fraction size of B-lymphocytes in the peripheral blood. We further examined if B-lymphogenesis is affected in the mice deficient for osteopontin subjected to HU. In bone marrow, HU decreased the population of the B-lymphocyte lineage cells significantly, whereas it did not alter the population of monocyte/macrophage lineage cells. HU also increased the cells in T-lymphocyte lineage in bone marrow. Interestingly, these changes were observed similarly both in OPN-deficient and wild-type mice. These results indicate for the first time that HU increases OPN expression in circulating cells and suppresses bone marrow B-lymphogenesis. | |
| 540 | |a The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 | ||
| 690 | 7 | |a Osteopontin |2 nationallicence | |
| 690 | 7 | |a Tail-suspension |2 nationallicence | |
| 690 | 7 | |a Hindlimb unloading |2 nationallicence | |
| 690 | 7 | |a PBMC |2 nationallicence | |
| 690 | 7 | |a Lymphocyte fraction |2 nationallicence | |
| 700 | 1 | |a Ezura |D Yoichi |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Nagata |D Junji |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Nagao |D Masashi |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Hemmi |D Hiroaki |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Hayata |D Tadayoshi |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | |
| 700 | 1 | |a Rittling |D Susan |u The Forsyth Institute, The Fenway, 02115, Boston, MA, USA |4 aut | |
| 700 | 1 | |a Denhardt |D David |u Department of Cell Biology and Neuroscience, Rutgers University, 08854, Piscataway, NJ, USA |4 aut | |
| 700 | 1 | |a Noda |D Masaki |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | |
| 773 | 0 | |t Journal of Bone and Mineral Metabolism |d Springer Japan |g 33/1(2015-01-01), 48-54 |x 0914-8779 |q 33:1<48 |1 2015 |2 33 |o 774 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00774-014-0568-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00774-014-0568-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ezura |D Yoichi |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nagata |D Junji |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nagao |D Masashi |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hemmi |D Hiroaki |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hayata |D Tadayoshi |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rittling |D Susan |u The Forsyth Institute, The Fenway, 02115, Boston, MA, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Denhardt |D David |u Department of Cell Biology and Neuroscience, Rutgers University, 08854, Piscataway, NJ, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Noda |D Masaki |u Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Bone and Mineral Metabolism |d Springer Japan |g 33/1(2015-01-01), 48-54 |x 0914-8779 |q 33:1<48 |1 2015 |2 33 |o 774 | ||