caa a22 4500 605462860 CHVBK 20210128100250.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00774-014-0568-8 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0568-8 Hindlimb-unloading suppresses B cell population in the bone marrow and peripheral circulation associated with OPN expression in circulating blood cells [Elektronische Daten] [Yoichi Ezura, Junji Nagata, Masashi Nagao, Hiroaki Hemmi, Tadayoshi Hayata, Susan Rittling, David Denhardt, Masaki Noda] Rodent hindlimb unloading (HU) by tail-suspension is a model to investigate disuse-induced bone loss in vivo. Previously, we have shown that osteopontin (OPN, also known as Spp1) is required for unloading-induced bone loss. However, how unloading affects OPN expression in the body is not fully understood. Here, we examined OPN expression in peripheral blood of mice subjected to HU. Real-time RT-PCR analysis indicated that OPN expression is increased in circulating peripheral blood cells. This HU-induced increase in OPN mRNA expression was specific in circulating peripheral blood cells, as OPN was not increased in the blood cells in bone marrow. HU-induced enhancement in OPN expression in peripheral blood cells was associated with an increase in the fraction of monocyte/macrophage lineage cells in the peripheral blood. In contrast, HU decreased the fraction size of B-lymphocytes in the peripheral blood. We further examined if B-lymphogenesis is affected in the mice deficient for osteopontin subjected to HU. In bone marrow, HU decreased the population of the B-lymphocyte lineage cells significantly, whereas it did not alter the population of monocyte/macrophage lineage cells. HU also increased the cells in T-lymphocyte lineage in bone marrow. Interestingly, these changes were observed similarly both in OPN-deficient and wild-type mice. These results indicate for the first time that HU increases OPN expression in circulating cells and suppresses bone marrow B-lymphogenesis. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Osteopontin nationallicence Tail-suspension nationallicence Hindlimb unloading nationallicence PBMC nationallicence Lymphocyte fraction nationallicence Ezura Yoichi Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut Nagata Junji Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut Nagao Masashi Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut Hemmi Hiroaki Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut Hayata Tadayoshi Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut Rittling Susan The Forsyth Institute, The Fenway, 02115, Boston, MA, USA aut Denhardt David Department of Cell Biology and Neuroscience, Rutgers University, 08854, Piscataway, NJ, USA aut Noda Masaki Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 48-54 0914-8779 33:1<48 2015 33 774 https://doi.org/10.1007/s00774-014-0568-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0568-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ezura Yoichi Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut NATIONALLICENCE 700 1- Nagata Junji Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut NATIONALLICENCE 700 1- Nagao Masashi Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut NATIONALLICENCE 700 1- Hemmi Hiroaki Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut NATIONALLICENCE 700 1- Hayata Tadayoshi Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut NATIONALLICENCE 700 1- Rittling Susan The Forsyth Institute, The Fenway, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Denhardt David Department of Cell Biology and Neuroscience, Rutgers University, 08854, Piscataway, NJ, USA aut NATIONALLICENCE 700 1- Noda Masaki Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 5-45 Yushima 1-Chome, Bunkyo-ku, 113-8510, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 48-54 0914-8779 33:1<48 2015 33 774