caa a22 4500 605462879 CHVBK 20210128100250.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00774-013-0557-3 doi (NATIONALLICENCE)springer-10.1007/s00774-013-0557-3 Methodology developed for the simultaneous measurement of bone formation and bone resorption in rats based on the pharmacokinetics of fluoride [Elektronische Daten] [Maela Lupo, Maria Brance, Brenda Fina, Lucas Brun, Alfredo Rigalli] This paper describes a novel methodology for the simultaneous estimation of bone formation (BF) and resorption (BR) in rats using fluoride as a nonradioactive bone-seeker ion. The pharmacokinetics of flouride have been extensively studied in rats; its constants have all been characterized. This knowledge was the cornerstone for the underlying mathematical model that we used to measure bone fluoride uptake and elimination rate after a dose of fluoride. Bone resorption and formation were estimated by bone fluoride uptake and elimination rate, respectively. ROC analysis showed that sensitivity, specificity and area under the ROC curve were not different from deoxypiridinoline and bone alkaline phosphatase, well-known bone markers. Sprague-Dawley rats with modified bone remodelling (ovariectomy, hyper, and hypocalcic diet, antiresorptive treatment) were used to validate the values obtained with this methodology. The results of BF and BR obtained with this technique were as expected for each biological model. Although the method should be performed under general anesthesia, it has several advantages: simultaneous measurement of BR and BF, low cost, and the use of compounds with no expiration date. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Bone remodeling nationallicence Mathematical model nationallicence Fluoride pharmacokinetics nationallicence Bone marker nationallicence Rat nationallicence Lupo Maela Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut Brance Maria Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut Fina Brenda Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut Brun Lucas Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut Rigalli Alfredo Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 16-22 0914-8779 33:1<16 2015 33 774 https://doi.org/10.1007/s00774-013-0557-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-013-0557-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lupo Maela Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut NATIONALLICENCE 700 1- Brance Maria Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut NATIONALLICENCE 700 1- Fina Brenda Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut NATIONALLICENCE 700 1- Brun Lucas Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut NATIONALLICENCE 700 1- Rigalli Alfredo Bone Biology Laboratory, School of Medicine, Rosario National University, Santa Fe 3100, Rosario, Santa Fe, Argentina aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 16-22 0914-8779 33:1<16 2015 33 774