caa a22 4500 605462895 CHVBK 20210128100250.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00774-014-0563-0 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0563-0 Effects of manganese deficiency on chondrocyte development in tibia growth plate of Arbor Acres chicks [Elektronische Daten] [Jian Wang, Zhen Wang, Zhao Wang, Ran Liu, Shao Liu, Lin Wang] The aim of this study was to investigate the effects of manganese (Mn) deficiency on chondrocyte development in tibia growth plate. Ninety 1-day-old Arbor Acres chicks were randomly divided into three groups and fed on control diet (60mg Mn/kg diet) and manganese deficient diets (40mg Mn/kg diet, manganese deficiency group I; 8.7mg Mn/kg diet, manganese deficiency group II), respectively. The width of the proliferative zone of growth plate was measured by the microscope graticule. Chondrocyte apoptosis was estimated by TUNEL staining. Gene expression of p21 and Bcl-2, and expression of related proteins were analyzed by quantitative real time reverse transcription polymerase chain reaction and immunohistochemistry, respectively. Compared with the control group, manganese deficiency significantly decreased the proliferative zone width and Bcl-2 mRNA expression level, while significantly increased the apoptotic rates and the expression level of p21 gene in chondrocytes. The results indicate that manganese deficiency had a negative effect on chondrocyte development, which was mediated by the inhibition of chondrocyte proliferation and promotion of chondrocyte apoptosis. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Manganese deficiency nationallicence Chondrocytes nationallicence Proliferation nationallicence Apoptosis nationallicence Wang Jian College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut Wang Zhen College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut Wang Zhao College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut Liu Ran College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut Liu Shao College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut Wang Lin College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 23-29 0914-8779 33:1<23 2015 33 774 https://doi.org/10.1007/s00774-014-0563-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0563-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jian College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhen College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhao College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut NATIONALLICENCE 700 1- Liu Ran College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut NATIONALLICENCE 700 1- Liu Shao College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut NATIONALLICENCE 700 1- Wang Lin College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, 271018, Taian, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 23-29 0914-8779 33:1<23 2015 33 774