caa a22 4500 60546295X CHVBK 20210128100251.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00774-014-0562-1 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0562-1 Development of new criteria for cortical bone histomorphometry in femoral neck: intra- and inter-observer reproducibility [Elektronische Daten] [Xiao-Yu Tong, Markus Malo, Inari Tamminen, Hanna Isaksson, Jukka Jurvelin, Heikki Kröger] Histomorphometry is commonly applied to study bone remodeling. Histological definitions of cortical bone boundaries have not been consistent. In this study, new criteria for specific definition of the transitional zone between the cortical and cancellous bone in the femoral neck were developed. The intra- and inter-observer reproducibility of this method was determined by quantitative histomorphometry and areal overlapping analysis. The undecalcified histological sections of femoral neck specimens (n=6; from men aged 17-59years) were processed and scanned to acquire histological images of complete bone sections. Specific criteria were applied to define histological boundaries. "Absolute cortex area” consisted of pure cortical bone tissue only, and was defined mainly based on the size of composite canals and their distance to an additional "guide” boundary (so-called "preliminary cortex boundary,” the clear demarcation line of density between compact cortex and sparse trabeculae). Endocortical bone area was defined by recognizing characteristic endocortical structures adjacent to the preliminary cortical boundary. The present results suggested moderate to high reproducibility for low-magnification parameters (e.g., cortical bone area). The coefficient of variation (CV %) ranged from 0.02 to 5.61 in the intra-observer study and from 0.09 to 16.41 in the inter-observer study. However, the intra-observer reproducibility of some high-magnification parameters (e.g., osteoid perimeter/endocortical perimeter) was lower (CV %, 0.33-87.9). The overlapping of three histological areas in repeated analyses revealed highest intra- and inter-observer reproducibility for the absolute cortex area. This study provides specific criteria for the definition of histological boundaries for femoral neck bone specimens, which may aid more precise cortical bone histomorphometry. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Cortical bone histomorphometry nationallicence Boundary definition nationallicence Reproducibility nationallicence Intra-observer study nationallicence Inter-observer study nationallicence Tong Xiao-Yu Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Malo Markus Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Tamminen Inari Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Isaksson Hanna Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Jurvelin Jukka Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Kröger Heikki Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 109-118 0914-8779 33:1<109 2015 33 774 https://doi.org/10.1007/s00774-014-0562-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0562-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tong Xiao-Yu Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Malo Markus Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Tamminen Inari Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Isaksson Hanna Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Jurvelin Jukka Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Kröger Heikki Bone and Cartilage Research Unit (BCRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/1(2015-01-01), 109-118 0914-8779 33:1<109 2015 33 774