caa a22 4500 605463220 CHVBK 20210128100252.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00774-014-0599-1 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0599-1 Raman spectroscopy delineates radiation-induced injury and partial rescue by amifostine in bone: a murine mandibular model [Elektronische Daten] [Peter Felice, Bo Gong, Salman Ahsan, Sagar Deshpande, Noah Nelson, Alexis Donneys, Catherine Tchanque-Fossuo, Michael Morris, Steven Buchman] Despite its therapeutic role in head and neck cancer, radiation administration degrades the biomechanical properties of bone and can lead to pathologic fracture and osteoradionecrosis. Our laboratories have previously demonstrated that prophylactic amifostine administration preserves the biomechanical properties of irradiated bone and that Raman spectroscopy accurately evaluates bone composition ex vivo. As such, we hypothesize that Raman spectroscopy can offer insight into the temporal and mechanical effects of both irradiation and amifostine administration on bone to potentially predict and even prevent radiation-induced injury. Male Sprague-Dawley rats (350-400g) were randomized into control, radiation exposure (XRT), and amifostine pre-treatment/radiation exposure groups (AMF-XRT). Irradiated animals received fractionated 70Gy radiation to the left hemi-mandible, while AMF-XRT animals received amifostine just prior to radiation. Hemi-mandibles were harvested at 18weeks after radiation, analyzed via Raman spectroscopy, and compared with specimens previously harvested at 8weeks after radiation. Mineral (ρ958) and collagen (ρ1665) depolarization ratios were significantly lower in XRT specimens than in AMF-XRT and control specimens at both 8 and 18weeks. amifostine administration resulted in a full return of mineral and collagen depolarization ratios to normal levels at 18weeks. Raman spectroscopy demonstrates radiation-induced damage to the chemical composition and ultrastructure of bone while amifostine prophylaxis results in a recovery towards normal, native mineral and collagen composition and orientation. These findings have the potential to impact on clinical evaluations and interventions by preventing or detecting radiation-induced injury in patients requiring radiotherapy as part of a treatment regimen. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Raman spectroscopy nationallicence Amifostine nationallicence Mandible nationallicence Pathologic fracture nationallicence Osteoradionecrosis nationallicence Felice Peter Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut Gong Bo Department of Chemistry, University of Michigan, Ann Arbor, USA aut Ahsan Salman Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut Deshpande Sagar Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut Nelson Noah Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut Donneys Alexis Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut Tchanque-Fossuo Catherine Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut Morris Michael Department of Chemistry, University of Michigan, Ann Arbor, USA aut Buchman Steven Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut Journal of Bone and Mineral Metabolism Springer Japan 33/3(2015-05-01), 279-284 0914-8779 33:3<279 2015 33 774 https://doi.org/10.1007/s00774-014-0599-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0599-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Felice Peter Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Gong Bo Department of Chemistry, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Ahsan Salman Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Deshpande Sagar Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Nelson Noah Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Donneys Alexis Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Tchanque-Fossuo Catherine Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Morris Michael Department of Chemistry, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 700 1- Buchman Steven Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan, Ann Arbor, USA aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/3(2015-05-01), 279-284 0914-8779 33:3<279 2015 33 774