caa a22 4500 605463247 CHVBK 20210128100252.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00774-014-0593-7 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0593-7 Osteocalcin, but not deoxypyridinoline, increases in response to isoflurane-induced anaesthesia in young female guinea pigs [Elektronische Daten] [Negar Tabatabaei, Celia Rodd, Richard Kremer, Hope Weiler] The effect of the inhaled anaesthetic isoflurane was investigated on bone biomarkers, both during maturation and on minerals and glucose postpartum. Female guinea pigs (n=10) were anaesthetized during maturation (5 and 9weeks) and postpartum (26weeks of age) with isoflurane during dual-energy X-ray absorptiometry scanning. Blood collection was performed at all ages before and after anaesthesia for measurement of plasma osteocalcin (OC), total deoxypyridinoline (tDPD), and cortisol. Postpartum measurements also included: blood ions, acid-base parameters and glucose, plasma minerals, total alkaline phosphatase (tALP), and albumin. Plasma OC concentration almost doubled after exposure to isoflurane at 5weeks (30.1±5.0-57.9±11.2nmol/L, p<0.001) and at 9weeks (29.1±7.5-62.9±15.9nmol/L, p<0.001), but did not change postpartum (3.7±3.3-4.3±3.9nmol/L, p=0.88). There was no effect of isoflurane exposure on plasma tDPD at any age. Plasma cortisol increased after exposure to isoflurane at 9weeks (1859.6±383.2-2748.0±235.3nmol/L, p<0.01) and postpartum (3376.7±322.2-4091.6±195.6nmol/L, p<0.001) but not at 5weeks (2088.3±326.4-2464.1±538.0nmol/L, p>0.05). Blood ionized Ca2+, Na+ and plasma total Ca did not change, whereas plasma albumin decreased, and inorganic phosphate (PO4) and Cl− increased upon exposure to isoflurane. Isoflurane decreased tALP (43.2±6.6-40.2±5.9IU/L, p=0.01) and increased glucose (7.5±0.6-10.9±1.7mmol/L, p<0.0001) postpartum. Isoflurane inflates the assessment of a bone-derived biomarker, OC, during rapid growth, but not following pregnancy when formation is very low. Measurements prior to anaesthesia are recommended to reflect normal metabolism. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Isoflurane nationallicence Osteocalcin nationallicence Deoxypyridinoline nationallicence Guinea pig nationallicence Tabatabaei Negar School of Dietetics and Human Nutrition, Macdonald-Stewart Building Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Quebec, Canada aut Rodd Celia Department of Pediatrics, Montreal Children's Hospital, McGill University Health Centre, 2300 Tupper St., H3H 1P3, Montreal, QC, Canada aut Kremer Richard Department of Medicine, Royal Victoria Hospital, McGill University Health Center, 687 Pine Avenue West, H3A 1A1, Montreal, QC, Canada aut Weiler Hope School of Dietetics and Human Nutrition, Macdonald-Stewart Building Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Quebec, Canada aut Journal of Bone and Mineral Metabolism Springer Japan 33/3(2015-05-01), 253-260 0914-8779 33:3<253 2015 33 774 https://doi.org/10.1007/s00774-014-0593-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0593-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tabatabaei Negar School of Dietetics and Human Nutrition, Macdonald-Stewart Building Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Quebec, Canada aut NATIONALLICENCE 700 1- Rodd Celia Department of Pediatrics, Montreal Children's Hospital, McGill University Health Centre, 2300 Tupper St., H3H 1P3, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Kremer Richard Department of Medicine, Royal Victoria Hospital, McGill University Health Center, 687 Pine Avenue West, H3A 1A1, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Weiler Hope School of Dietetics and Human Nutrition, Macdonald-Stewart Building Macdonald Campus, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, H9X 3V9, Quebec, Canada aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/3(2015-05-01), 253-260 0914-8779 33:3<253 2015 33 774