caa a22 4500 605463379 CHVBK 20210128100253.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00774-014-0607-5 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0607-5 Effect of lysophosphatidic acid receptor inhibition on bone changes in ovariectomized mice [Elektronische Daten] [Beatriz Orosa, Paula Martínez, Antonio González, David Guede, José Caeiro, Juan Gómez-Reino, Carmen Conde] Pharmacological inhibition of signaling through lysophosphatidic acid (LPA) receptors reduces bone erosions in an experimental model of arthritis by mechanisms involving reduced osteoclast differentiation and bone resorption and increased differentiation of osteoblasts and bone mineralization. These results led us to hypothesize that LPA receptor inhibition would be beneficial in osteoporosis. Our aim was to test this hypothesis with the LPA receptor antagonist, Ki16425, in ovariectomized mice, a model of postmenopausal osteoporosis. Ovariectomized mice treated with Ki16425 showed bone loss similar to that observed in the controls. Osteoblast markers, Alpl, Bglap and Col1a1, were increased at the mRNA level but no changes were detected in serum. No additional difference was observed in the Ki16425-treated mice relative to the ovariectomized controls with regard to osteoclast function markers or assays of matrix mineralization or osteoclast differentiation. Thus, pharmacological inhibition of LPA receptor was not beneficial for preventing bone loss in ovariectomized mice, indicating that its favorable effect on bone remodeling is less general than hypothesized. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Lysophosphatidic acid receptor nationallicence Osteoporosis nationallicence Osteoclasts nationallicence Osteoblasts nationallicence Ovariectomy nationallicence Orosa Beatriz Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut Martínez Paula Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut González Antonio Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut Guede David Trabeculae S.L., Technological Park of Galicia, 32900, Ourense, Spain aut Caeiro José Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain aut Gómez-Reino Juan Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut Conde Carmen Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut Journal of Bone and Mineral Metabolism Springer Japan 33/4(2015-07-01), 383-391 0914-8779 33:4<383 2015 33 774 https://doi.org/10.1007/s00774-014-0607-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0607-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Orosa Beatriz Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Martínez Paula Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- González Antonio Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Guede David Trabeculae S.L., Technological Park of Galicia, 32900, Ourense, Spain aut NATIONALLICENCE 700 1- Caeiro José Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain aut NATIONALLICENCE 700 1- Gómez-Reino Juan Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Conde Carmen Laboratorio de Investigación 8 y Servicio de Reumatología, Instituto de Investigación Sanitaria (IDIS), Hospital Clínico Universitario de Santiago de Compostela (CHUS), SERGAS, Travesía da Choupana s/n, 15706, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/4(2015-07-01), 383-391 0914-8779 33:4<383 2015 33 774