caa a22 4500 605463387 CHVBK 20210128100253.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00774-014-0601-y doi (NATIONALLICENCE)springer-10.1007/s00774-014-0601-y A comparison of the in vitro mineralisation and dentinogenic potential of mesenchymal stem cells derived from adipose tissue, bone marrow and dental pulp [Elektronische Daten] [O. Davies, P. Cooper, R. Shelton, A. Smith, B. Scheven] Stem-cell-based therapies provide a biological basis for the regeneration of mineralised tissues. Stem cells isolated from adipose tissue (ADSCs), bone marrow (BMSCs) and dental pulp (DPSCs) have the capacity to form mineralised tissue. However, studies comparing the capacity of ADSCs with BMSCs and DPSCs for mineralised tissue engineering are lacking, and their ability to regenerate dental tissues has not been fully explored. Characterisation of the cells using fluorescence-activated cell sorting and semi-quantitative reverse transcription PCR for MSC markers indicated that they were immunophenotypically similar. Alizarin red (AR) staining and micro-computed tomography (µCT) analyses demonstrated that the osteogenic potential of DPSCs was significantly greater than that of BMSCs and ADSCs. Scanning electron microscopy and AR staining showed that the pattern of mineralisation in DPSC cultures differed from ADSCs and BMSCs, with DPSC cultures lacking defined mineralised nodules and instead forming a diffuse layer of low-density mineral. Dentine matrix components (DMCs) were used to promote dentinogenic differentiation. Their addition to cultures resulted in increased amounts of mineral deposited in all three cultures and significantly increased the density of mineral deposited in BMSC cultures, as determined by µCT analysis. Addition of DMCs also increased the relative gene expression levels of the dentinogenic markers dentine sialophosphoprotein and dentine matrix protein 1 in ADSC and BMSC cultures. In conclusion, DPSCs show the greatest potential to produce a comparatively high volume of mineralised matrix; however, both dentinogenesis and mineral volume was enhanced in ADSC and BMSC cultures by DMCs, suggesting that these cells show promise for regenerative dental therapies. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 MSC nationallicence Mineralisation nationallicence Dentine nationallicence Micro-computed tomography nationallicence Davies O. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut Cooper P. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut Shelton R. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut Smith A. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut Scheven B. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut Journal of Bone and Mineral Metabolism Springer Japan 33/4(2015-07-01), 371-382 0914-8779 33:4<371 2015 33 774 https://doi.org/10.1007/s00774-014-0601-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0601-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Davies O. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut NATIONALLICENCE 700 1- Cooper P. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut NATIONALLICENCE 700 1- Shelton R. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut NATIONALLICENCE 700 1- Smith A. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut NATIONALLICENCE 700 1- Scheven B. School of Dentistry, University of Birmingham, St Chad's Queensway, B4 6NN, Birmingham, UK aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/4(2015-07-01), 371-382 0914-8779 33:4<371 2015 33 774