caa a22 4500 605463530 CHVBK 20210128100253.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00774-014-0622-6 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0622-6 What is the role of bosentan in healing of femur fractures in a rat model? [Elektronische Daten] [Ali Aydin, Zekai Halici, Erol Akpinar, A. Aksakal, Murat Saritemur, Muhammed Yayla, C. Kunak, Elif Cadirci, H. Atmaca, S. Karcioglu] The purpose of this study was to examine the effects bosentan (which is a strong vasoconstrictor) on bone fracture pathophysiology, and investigate the roles of the nonselective endothelin1 receptor blocker bosentan on the bone fractures formed in rats through radiographic, histopathologic, and immunohistochemical methods. The rats were divided into three groups (six rats in each group): a femoral fracture control group, a femoral fractureplus bosentan at 50mg/kg group, and a femoral fracture plus bosentan at 100mg/kg group. The femoral fracture model was established by transversely cutting the femur at the midsection. After manual reduction, the fractured femur was fixed with intramedullary Kirschner wires. The radiographic healing scores of the bosentan 100 and 50mg/kg groups were significantly better that those of the fracture control group. The fracture callus percent of new bone in the bosentan 100mg/kg group was significantly greater than that in the control group. Also, semiquantitative analysis showed higher positive vascular endothelial growth factor and osteocalcin staining and lower positive endothelinreceptor typeA staining in the treatment groups than in the control group. Bosentan treatment also decreased tissue endothelin1 expression relative to that in the fracture control group. As a result of our study, the protective effect of bosentan was shown in experimental femoral fracture healing in rats by radiographic, histopathologic, and molecular analyses. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Bosentan nationallicence Endothelin1 nationallicence Bone fracture nationallicence Rat nationallicence Femur nationallicence Aydin Ali Department of Orthopedics and Traumatology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut Halici Zekai Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut Akpinar Erol Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut Aksakal A. Department of Orthopedics and Traumathology, Sevket Yilmaz Education and Research Hospital, 16230, Bursa, Turkey aut Saritemur Murat Department of Emergency Medicine, Ataturk University Faculty of Pharmacy, 25240, Erzurum, Turkey aut Yayla Muhammed Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut Kunak C. Department of Pharmacology, Ordu University Faculty of Medicine, 52240, Ordu, Turkey aut Cadirci Elif Department of Pharmacology, Ataturk University Faculty of Pharmacy, 25240, Erzurum, Turkey aut Atmaca H. Department of Pathology, Kırıkkale University Faculty of Veterinary, 71240, Kırıkkale, Turkey aut Karcioglu S. Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut Journal of Bone and Mineral Metabolism Springer Japan 33/5(2015-09-01), 496-506 0914-8779 33:5<496 2015 33 774 https://doi.org/10.1007/s00774-014-0622-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0622-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aydin Ali Department of Orthopedics and Traumatology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Halici Zekai Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Akpinar Erol Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Aksakal A. Department of Orthopedics and Traumathology, Sevket Yilmaz Education and Research Hospital, 16230, Bursa, Turkey aut NATIONALLICENCE 700 1- Saritemur Murat Department of Emergency Medicine, Ataturk University Faculty of Pharmacy, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Yayla Muhammed Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Kunak C. Department of Pharmacology, Ordu University Faculty of Medicine, 52240, Ordu, Turkey aut NATIONALLICENCE 700 1- Cadirci Elif Department of Pharmacology, Ataturk University Faculty of Pharmacy, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Atmaca H. Department of Pathology, Kırıkkale University Faculty of Veterinary, 71240, Kırıkkale, Turkey aut NATIONALLICENCE 700 1- Karcioglu S. Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/5(2015-09-01), 496-506 0914-8779 33:5<496 2015 33 774