caa a22 4500 605463603 CHVBK 20210128100254.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00774-014-0578-6 doi (NATIONALLICENCE)springer-10.1007/s00774-014-0578-6 Effect of Urocortin on strength and microarchitecture of osteopenic rat femur [Elektronische Daten] [Mohammad Tezval, Silja Hansen, Ulrich Schmelz, Marina Komrakova, Klaus Stuermer, Stephan Sehmisch] As yet there is no evidence of the potential antiosteoporotic effect of Urocortin-1 (UCN), a corticotropin releasing factor related peptide, in vivo. In this study, and for the first time, we investigated the effect of UCN in a rat osteopenia model. Sixty female Sprague-Dawley rats were divided into 5groups: (1) sham-operated, (2) untreated ovariectomized (OVX) rats, (3) and (4) OVX animals treated for 5weeks with daily subcutaneous low-dose UCN (3μg/kg of BW) or high-dose UCN (30μg/kg of BW) 8weeks after ovariectomy, and (5) OVX rats treated with daily estrogen (0.2mg/kg of BW p.o) 8weeks after ovariectomy for 5weeks (E). After sacrifice, the femurs were reserved for biomechanical, histomorphometric and ash testing. In the biomechanical test, the high-dose UCN rats showed significantly improved mechanical stiffness (341.6N/mm) compared with the untreated OVX animals (275.9N/mm). In the histomorphometric evaluation, the high-dose UCN rats demonstrated an improved trabecular microarchitecture especially and significantly at the distal femur (distal femur Tb.Ar=41.4% and N.Nd/mm2=26.8, proximal femur Tb.Ar=71.8% and N.Nd/mm2=28.7) compared with untreated OVX rats (distal femur Tb.Ar=23.3% and N.Nd/mm2=11.7, proximal femur Tb.Ar=60.2% and N.Nd/mm2=25.2). Our results show that short-term treatment with UCN seems to have a positive effect on the metaphyseal bone structure and strength of the femur in ovariectomized rats. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Urocortin-1 nationallicence Osteoporosis nationallicence Femur nationallicence Ovariectomized rat nationallicence Bone quality nationallicence Tezval Mohammad Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut Hansen Silja Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut Schmelz Ulrich Medical Institute of General Hygiene and Environmental Health, University of Goettingen, Goettingen, Germany aut Komrakova Marina Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut Stuermer Klaus Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut Sehmisch Stephan Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut Journal of Bone and Mineral Metabolism Springer Japan 33/2(2015-03-01), 154-160 0914-8779 33:2<154 2015 33 774 https://doi.org/10.1007/s00774-014-0578-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0578-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tezval Mohammad Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut NATIONALLICENCE 700 1- Hansen Silja Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut NATIONALLICENCE 700 1- Schmelz Ulrich Medical Institute of General Hygiene and Environmental Health, University of Goettingen, Goettingen, Germany aut NATIONALLICENCE 700 1- Komrakova Marina Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut NATIONALLICENCE 700 1- Stuermer Klaus Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut NATIONALLICENCE 700 1- Sehmisch Stephan Department of Trauma and Reconstructive Surgery, Georg-August-University of Goettingen, Goettingen, Germany aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/2(2015-03-01), 154-160 0914-8779 33:2<154 2015 33 774