caa a22 4500 60546362X CHVBK 20210128100254.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00774-014-0574-x doi (NATIONALLICENCE)springer-10.1007/s00774-014-0574-x Inhibitory effect of bisphosphonate on osteoclast function contributes to improved skeletal pain in ovariectomized mice [Elektronische Daten] [Yasuhisa Abe, Kousuke Iba, Koichi Sasaki, Hironori Chiba, Kumiko Kanaya, Tomoyuki Kawamata, Kimimitsu Oda, Norio Amizuka, Muneteru Sasaki, Toshihiko Yamashita] The aim of this study was to evaluate skeletal pain associated with osteoporosis and to examine the inhibitory effect of bisphosphonate (BP) on pain in an ovariectomized (OVX) mouse model. We evaluated skeletal pain in OVX mice through an examination of pain-like behavior as well as immunohistochemical findings. In addition, we assessed the effects of alendronate (ALN), a potent osteoclast inhibitor, on those parameters. The OVX mice showed a decrease in the pain threshold value, and an increase in the number of c-Fos immunoreactive neurons in laminae I-II of the dorsal horn of the spinal cord. Alendronate caused an increase in the pain threshold value and inhibited c-Fos expression. The serum level of tartrate-resistant acid phosphatase 5b, a marker of osteoclast activity, was significantly negatively correlated with the pain threshold value. Furthermore, we found that an antagonist of the transient receptor potential channel vanilloid subfamily member 1, which is an acid-sensing nociceptor, improved pain-like behavior in OVX mice. These results indicated that the inhibitory effect of BP on osteoclast function might contribute to an improvement in skeletal pain in osteoporosis patients. The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 Pain nationallicence Osteoporosis nationallicence Osteoclast nationallicence Bisphosphonate nationallicence Transient receptor potential channel vanilloid subfamily member 1 (TRPV1) nationallicence Abe Yasuhisa Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut Iba Kousuke Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut Sasaki Koichi Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut Chiba Hironori Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut Kanaya Kumiko Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut Kawamata Tomoyuki Department of Anesthesiology and Resuscitology, Shinsyu University School of Medicine, Matsumoto, Japan aut Oda Kimimitsu Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan aut Amizuka Norio Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan aut Sasaki Muneteru Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan aut Yamashita Toshihiko Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut Journal of Bone and Mineral Metabolism Springer Japan 33/2(2015-03-01), 125-134 0914-8779 33:2<125 2015 33 774 https://doi.org/10.1007/s00774-014-0574-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00774-014-0574-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abe Yasuhisa Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut NATIONALLICENCE 700 1- Iba Kousuke Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut NATIONALLICENCE 700 1- Sasaki Koichi Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut NATIONALLICENCE 700 1- Chiba Hironori Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut NATIONALLICENCE 700 1- Kanaya Kumiko Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut NATIONALLICENCE 700 1- Kawamata Tomoyuki Department of Anesthesiology and Resuscitology, Shinsyu University School of Medicine, Matsumoto, Japan aut NATIONALLICENCE 700 1- Oda Kimimitsu Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan aut NATIONALLICENCE 700 1- Amizuka Norio Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan aut NATIONALLICENCE 700 1- Sasaki Muneteru Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan aut NATIONALLICENCE 700 1- Yamashita Toshihiko Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuo-ku, 060-8543, Sapporo, Japan aut NATIONALLICENCE 773 0- Journal of Bone and Mineral Metabolism Springer Japan 33/2(2015-03-01), 125-134 0914-8779 33:2<125 2015 33 774