Effects of interleukin-11 on the expression of human bone sialoprotein gene
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| 024 | 7 | 0 | |a 10.1007/s00774-014-0576-8 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00774-014-0576-8 | ||
| 245 | 0 | 0 | |a Effects of interleukin-11 on the expression of human bone sialoprotein gene |h [Elektronische Daten] |c [Hiroyoshi Matsumura, Yohei Nakayama, Hideki Takai, Yorimasa Ogata] |
| 520 | 3 | |a Interleukin-11 (IL-11) is a bone marrow stromal fibroblast-derived cytokine with a wide spectrum of activities in different biological systems. IL-11 and IL-6 are two cytokines known to rely on osteoblast-osteoclast communication for their effects on osteoclast differentiation. Bone sialoprotein (BSP) is a mineralized connective tissue-specific protein expressed in differentiated osteoblasts, odontoblasts, and cementoblasts. To determine the molecular basis of the transcriptional regulation of the human BSP gene by IL-11, we conducted real-time polymerase chain reactions (PCR), transient transfection analyses with chimeric constructs of the human BSP gene promoter linked to a luciferase reporter gene, gel mobility shift assays, and a chromatin immunoprecipitation assay using human osteoblast-like Saos2 cells. IL-11 (20ng/ml) increased BSP, Runx2, and Osterix mRNA levels at 6h and the alkaline phosphatase (ALP) mRNA level at 12h in osteoblast-like Saos2 cells. In a transient transfection assay, IL-11 (20ng/ml, 12h) increased luciferase activities of constructs between −60LUC and −868LUC including the human BSP gene promoter. Transcriptional stimulations by IL-11 were partially inhibited in the constructs that included 2-bp mutations in the cAMP response element 1 (CRE1, −72 to −79) and CRE2 (−667 to −674). When mutations were made in pairs of CRE1 and CRE2 in −868LUC, the effect of IL-11 on luciferase activity was almost totally abrogated. Transcriptional activities induced by IL-11 were inhibited by protein kinase A, tyrosine kinase, ERK1/2, and PI3-kinase inhibitors. Gel mobility shift analyses showed that IL-11 increased nuclear proteins binding to CRE1 and CRE2. CREB1, phospho-CREB1, c-Fos, and c-Jun antibodies disrupted the formation of CRE1 and CRE2 protein complexes. These data demonstrate that IL-11 stimulates BSP gene transcription via CRE1 and CRE2 elements in the human BSP gene promoter. | |
| 540 | |a The Japanese Society for Bone and Mineral Research and Springer Japan, 2014 | ||
| 690 | 7 | |a Bone sialoprotein |2 nationallicence | |
| 690 | 7 | |a Interleukin-11 |2 nationallicence | |
| 690 | 7 | |a Osteoblasts |2 nationallicence | |
| 690 | 7 | |a Transcription |2 nationallicence | |
| 700 | 1 | |a Matsumura |D Hiroyoshi |u Department of Periodontology, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | |
| 700 | 1 | |a Nakayama |D Yohei |u Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | |
| 700 | 1 | |a Takai |D Hideki |u Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | |
| 700 | 1 | |a Ogata |D Yorimasa |u Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | |
| 773 | 0 | |t Journal of Bone and Mineral Metabolism |d Springer Japan |g 33/2(2015-03-01), 142-153 |x 0914-8779 |q 33:2<142 |1 2015 |2 33 |o 774 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00774-014-0576-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00774-014-0576-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Matsumura |D Hiroyoshi |u Department of Periodontology, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nakayama |D Yohei |u Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Takai |D Hideki |u Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ogata |D Yorimasa |u Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, 271-8587, Matsudo, Chiba, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Journal of Bone and Mineral Metabolism |d Springer Japan |g 33/2(2015-03-01), 142-153 |x 0914-8779 |q 33:2<142 |1 2015 |2 33 |o 774 | ||