caa a22 4500 605464057 CHVBK 20210128100256.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10499-014-9822-2 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9822-2 Effect of galangal ( Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp ( Litopenaeus vannamei ) [Elektronische Daten] [Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Lila Ruangpan, Kei Nagata, Kaeko Kamei] Some plant and herb extracts reportedly possess antimicrobial activities and also have the ability to enhance the nonspecific immune system of shrimp, thereby promoting disease resistance. In this study, a natural herb, galangal (Alpinia galanga Linn.), was used to prevent infectious disease in cultured shrimp, and the effects of galangal-ethanol extract on the expression of the immune-related genes in the shrimp were analyzed via a reverse-transcription polymerase chain reaction of mRNA in circulating hemocytes. Following the intramuscular injection of either galangal extract or trans-p-coumaryl diacetate that had been isolated from galangal rhizome, Pacific white shrimp (Litopenaeus vannamei) showed significant increases in the relative expression ratio of the six immune-related genes compared with a control group. Furthermore, following the oral administration of galangal extract, similar inducible effects of the expression of immune-related genes in the Pacific white shrimp were obtained, which led to an enhanced survival rate from Vibrio harveyi infection. Thus, this study revealed that both galangal extract and trans-p-coumaryl diacetate stimulated the immune system response, thereby promoting resistance to V. harveyi infection in Pacific white shrimp. Springer International Publishing Switzerland, 2014 Galangal ( Alpinia galanga Linn.) nationallicence Trans - p -coumaryl diacetate nationallicence Litopenaeus vannamei nationallicence Immunity nationallicence Vibrio nationallicence Chaweepack Tidaporn Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut Chaweepack Surachart Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut Muenthaisong Boonyee Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut Ruangpan Lila Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut Nagata Kei Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan aut Kamei Kaeko Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan aut Aquaculture International Springer International Publishing 23/1(2015-02-01), 385-399 0967-6120 23:1<385 2015 23 10499 https://doi.org/10.1007/s10499-014-9822-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9822-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaweepack Tidaporn Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut NATIONALLICENCE 700 1- Chaweepack Surachart Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut NATIONALLICENCE 700 1- Muenthaisong Boonyee Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut NATIONALLICENCE 700 1- Ruangpan Lila Chanthaburi Coastal Fisheries Research and Development Center, Mu 9, Bangkaja, Muang, 22000, Chanthaburi, Thailand aut NATIONALLICENCE 700 1- Nagata Kei Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan aut NATIONALLICENCE 700 1- Kamei Kaeko Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/1(2015-02-01), 385-399 0967-6120 23:1<385 2015 23 10499