caa a22 4500 60546409X CHVBK 20210128100256.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10499-014-9797-z doi (NATIONALLICENCE)springer-10.1007/s10499-014-9797-z The effects of stocking density on hemato-immunological and serum biochemical parameters of rainbow trout ( Oncorhynchus mykiss ) [Elektronische Daten] [Peyman Yarahmadi, Hamed Miandare, Seyed Hoseinifar, Nahid Gheysvandi, Arash Akbarzadeh] In the present study, the effect of stocking density on hematological, biochemical and immunological parameters of rainbow trout (Oncorhynchus mykiss) was investigated. Experiments were performed in a 30-day period at two different stocking densities: low density (LD 10kg/m3) and high density (HD 45kg/m3). Fish held at HD showed significant higher levels of hemoglobin and red blood cells (12.76mg/dl and 1.8×106mm3, respectively) compared with those held at LD (11.43mg/dl and 1.23×106mm3, respectively; P<0.05). Stocking density had no significant effect on mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration levels. The level of HCT was higher in fish reared at HD, but it was not statistically significant. In the case of biochemical parameters, plasma glucose and cortisol increased significantly in HD compared with LD (P<0.05), but stocking density caused no significant changes in total protein, globulin and albumin levels. Our results also revealed that the levels of plasma cholesterol and triglycerides were significantly lower in LD compared with HD (P<0.05). Results of some immune parameters of O. mykiss reared at two different stocking densities revealed that at HD, the level of white blood cells was lower significantly compared with LD (P<0.05). The percentage of lymphocytes, neutrophiles and monocytes showed no significant changes between two groups. In addition, the level of serum lysozyme was significantly higher in animals maintained at HD compared with LD (P<0.05). Overall, our results show that high stocking density caused chronic stress in rainbow trout and consequently altered the levels of some hematological, biochemical and immunological parameters compared with fish kept at low stocking density. Springer International Publishing Switzerland, 2014 Stocking density nationallicence Hematological nationallicence Biochemical and immunological parameters nationallicence Rainbow trout nationallicence Yarahmadi Peyman Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran aut Miandare Hamed Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739, Gorgan, Iran aut Hoseinifar Seyed Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739, Gorgan, Iran aut Gheysvandi Nahid Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739, Gorgan, Iran aut Akbarzadeh Arash Department of Fisheries, Faculty of Natural Resources, University of Hormozgan, Bandar Abbas, Iran aut Aquaculture International Springer International Publishing 23/1(2015-02-01), 55-63 0967-6120 23:1<55 2015 23 10499 https://doi.org/10.1007/s10499-014-9797-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9797-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yarahmadi Peyman Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran aut NATIONALLICENCE 700 1- Miandare Hamed Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739, Gorgan, Iran aut NATIONALLICENCE 700 1- Hoseinifar Seyed Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739, Gorgan, Iran aut NATIONALLICENCE 700 1- Gheysvandi Nahid Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, 49138-15739, Gorgan, Iran aut NATIONALLICENCE 700 1- Akbarzadeh Arash Department of Fisheries, Faculty of Natural Resources, University of Hormozgan, Bandar Abbas, Iran aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/1(2015-02-01), 55-63 0967-6120 23:1<55 2015 23 10499