caa a22 4500 605464111 CHVBK 20210128100256.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10499-014-9808-0 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9808-0 Experimental transmission of Macrobrachium rosenbergii nodavirus ( Mr NV) and extra small virus (XSV) in Macrobrachium malcolmsonii and Macrobrachium rude [Elektronische Daten] [M. Ravi, A. Sahul Hameed] The susceptibility of two species of freshwater prawn, Macrobrachium malcolmsonii (17.7±1.85g, 9.5±0.50cm) and Macrobrachium rude (4.14±0.95g, 4.1±0.56cm), to Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) was tested. These two species were artificially infected with MrNV and XSV by oral and intramuscular routes using viral inoculum prepared from white tail disease—infected post-larvae of M. rosenbergii. No mortality or clinical symptoms were observed in the experimentally infected M. rude and M. malcolmsonii. The data of experimental infection indicate that MrNV and XSV failed to cause the mortality in these two species of freshwater prawn. But RT-PCR analysis of different organs of experimentally infected prawns showed the presence of MrNV and XSV in all the organs analyzed. Further, the viral inoculum prepared from experimentally infected M. malcolmsonii and M. rude caused 100% mortality in post-larvae of M. rosenbergii (43.0±2mg, 1.34±0.01cm, PL stage 10). These results indicate the possibility of M. malcolmsonii and M. rude acting as reservoir for MrNV and XSV and maintaining their virulence in their tissues. Springer International Publishing Switzerland, 2014 Macrobrachium malcolmsonii nationallicence Macrobrachium rude nationallicence Host nationallicence WTD nationallicence Mr NV nationallicence XSV nationallicence Ravi M. Centre for Ocean Research, Sathyabama University, Jappiaar Nagar, Rajiv Gandhi Road, 600 119, Chennai, Tamil Nadu, India aut Sahul Hameed A. OIE Reference Laboratory for WTD, Department of Zoology, C. Abdul Hakeem College, 632509, Melvisharam, Tamil Nadu, India aut Aquaculture International Springer International Publishing 23/1(2015-02-01), 195-201 0967-6120 23:1<195 2015 23 10499 https://doi.org/10.1007/s10499-014-9808-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9808-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ravi M. Centre for Ocean Research, Sathyabama University, Jappiaar Nagar, Rajiv Gandhi Road, 600 119, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Sahul Hameed A. OIE Reference Laboratory for WTD, Department of Zoology, C. Abdul Hakeem College, 632509, Melvisharam, Tamil Nadu, India aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/1(2015-02-01), 195-201 0967-6120 23:1<195 2015 23 10499