caa a22 4500 605464138 CHVBK 20210128100256.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10499-014-9811-5 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9811-5 Effects of l -tryptophan-supplemented dietary on growth performance and 5-HT and GABA levels in juvenile Litopenaeus vannamei [Elektronische Daten] [Yu-Ping Sun, Li-Zeng Guan, Jie-Hua Xiong, Qian-Yun Xi, Yong-Liang Zhang] A 60-day feeding trial was conducted to evaluate the effects of dietary l-tryptophan level on the growth performance and neurotransmitter, including 5-hydroxytryptamine (5-HT) and γ-aminobutyric acid (GABA) of juvenile Litopenaeus vannamei. Six experimental diets were formulated to supplement with graded levels of tryptophan, resulting in different dietary tryptophan levels at 0 (control), 0.12, 0.24, 0.36, 0.48 and 0.60%, respectively. A total of 960 shrimps (0.63±0.01g) were divided into six treatments and fed with the respective tryptophan diets in an indoor recirculating system. The results showed that the body weight, body length, live weight gain, specific growth rate, protein efficiency ratio and survival rate were significantly increased by dietary tryptophan levels, and the maximum were all obtained at 0.60% tryptophan-supplemented diet. Feed conversion ratio was significantly decreased as dietary tryptophan level increased. No significant difference was found in hepatopancreas index and body proximate composition among all the treatments. High-performance liquid chromatography analysis showed that 5-HT level increased first and then decreased in all examined tissues except in thoracic ganglia with dietary tryptophan level increased, while GABA level first elevated and then decreased in X organ-sinus gland, brain, stomach and intestinal tract, and on the contrary, in thoracic ganglia, hepatopancreas, but declined in hemolymph with higher dietary tryptophan level. qRT-PCR results exhibited 5-HT1 mRNA expression was similar to 5-HT level variation. Based on these results, the dietary tryptophan supplemented 0.36% dry diet or 1.72% protein diet is found to be optimum to improve L. vannamei growth performance. In conclusion, it suggested that diet supplemented with tryptophan was beneficial to improve growth performance possibly by mediating 5-HT as well as GABA pathway in L. vannamei. These data provide new insight into the physiological functions of tryptophan in shrimp. Springer International Publishing Switzerland, 2014 Tryptophan nationallicence Growth performance nationallicence 5-HT nationallicence 5-HT1 receptor nationallicence GABA nationallicence Juvenile Litopenaeus vannamei nationallicence 5-HT : 5-Hydroxytryptamine, serotonin nationallicence GABA : γ-Aminobutyric acid nationallicence WG : Weight gain nationallicence SGR : Specific growth rate nationallicence FCR : Feed conversion ratio nationallicence PER : Protein efficiency ratio nationallicence HSI : Hepatosomatic index nationallicence XO-SG : X organ-sinus gland nationallicence Sun Yu-Ping College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut Guan Li-Zeng Agriculture College, Yanbian University, 133000, Yanji, China aut Xiong Jie-Hua College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut Xi Qian-Yun College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut Zhang Yong-Liang College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut Aquaculture International Springer International Publishing 23/1(2015-02-01), 235-251 0967-6120 23:1<235 2015 23 10499 https://doi.org/10.1007/s10499-014-9811-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9811-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Yu-Ping College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Guan Li-Zeng Agriculture College, Yanbian University, 133000, Yanji, China aut NATIONALLICENCE 700 1- Xiong Jie-Hua College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Xi Qian-Yun College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Yong-Liang College of Animal Science, SCAU-Alltech Research Joint Alliance, South China Agricultural University, 483 Wushan Road, 510642, Guangzhou, China aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/1(2015-02-01), 235-251 0967-6120 23:1<235 2015 23 10499