Study of the genomic constitution of the German mirror carp
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| 024 | 7 | 0 | |a 10.1007/s10499-014-9799-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10499-014-9799-x | ||
| 245 | 0 | 0 | |a Study of the genomic constitution of the German mirror carp |h [Elektronische Daten] |c [Ya-Juan Li, Bo Liu, He Zhou, Yang-Chun Gao, Min Gao, Yu-Sheng Wang, Ding-Chen Cao, Xiao-Wen Sun] |
| 520 | 3 | |a Semen was treated with ultraviolet irradiation to genetically inactivate the sperm. Gynogenetic haploidy was induced artificially in the German mirror carp, Cyprinus carpio L. A systematic study of the status of early embryonic development, the number of chromosomes, chromosomal banding patterns and fluorescent in situ hybridization (FISH), and a microsatellite analysis were performed. The fertility, hatchability, and survival rates of the gynogenetic group were significantly lower than those of the normal control fertilization group (P<0.05). Gynogenetic larval growth was abnormal and the embryos did not survive. The chromosome number of the gynogenetic group was 50, whereas that of the control group was 100. The gynogenetic group had a single silver-staining point, one CMA3/DA/DAPI-positive position, and a single FISH-positive signal, whereas the control group had two silver-staining points, two CMA3/DA/DAPI-staining positions, and two FISH-positive signals. The microsatellite analysis showed that all the genes of the gynogenetic group derived from the female parent and that the females generated a set of haploid (n=50) gametes during meiosis. Using chromosome manipulation, this study confirms that the German mirror carp has two sets of chromosomes and is an evolutionary tetraploid (2n=100). | |
| 540 | |a Springer International Publishing Switzerland, 2014 | ||
| 690 | 7 | |a Cyprinus carpio L |2 nationallicence | |
| 690 | 7 | |a Gynogenetic |2 nationallicence | |
| 690 | 7 | |a Ag-NORs |2 nationallicence | |
| 690 | 7 | |a CMA3/DA/DAPI |2 nationallicence | |
| 690 | 7 | |a Fluorescence in situ hybridization |2 nationallicence | |
| 690 | 7 | |a Microsatellite analysis |2 nationallicence | |
| 690 | 7 | |a A2 : Luteinizing hormone releasing hormone A2 for injection |2 nationallicence | |
| 690 | 7 | |a CMA3 : Chromomysin A3 |2 nationallicence | |
| 690 | 7 | |a DA : Distamycin A |2 nationallicence | |
| 690 | 7 | |a DAPI : 4′6-Deamidino-2-phenylindole |2 nationallicence | |
| 690 | 7 | |a DNA : Deoxyribonucleic acid |2 nationallicence | |
| 690 | 7 | |a dNTPs : Deoxynucleotide triphosphates |2 nationallicence | |
| 690 | 7 | |a 16-dUTP : Biotin-ε-aminocaproyl-γ-aminobutyryl-[5-(3-aminoallyl)-2′-deoxy-uridine-5′-triphate] tetralithium salt |2 nationallicence | |
| 690 | 7 | |a DOM : Dopamine antagonist |2 nationallicence | |
| 690 | 7 | |a FISH : Fluorescence in situ hybridization |2 nationallicence | |
| 690 | 7 | |a MI : First meiotic division |2 nationallicence | |
| 690 | 7 | |a NORs : Nucleolar organizer regions |2 nationallicence | |
| 690 | 7 | |a PCR : Polymerase chain reaction |2 nationallicence | |
| 690 | 7 | |a rDNA : Ribosomal deoxyribonucleic acid |2 nationallicence | |
| 690 | 7 | |a UV : Ultraviolet |2 nationallicence | |
| 700 | 1 | |a Li |D Ya-Juan |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | |
| 700 | 1 | |a Liu |D Bo |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | |
| 700 | 1 | |a Zhou |D He |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | |
| 700 | 1 | |a Gao |D Yang-Chun |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | |
| 700 | 1 | |a Gao |D Min |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | |
| 700 | 1 | |a Wang |D Yu-Sheng |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | |
| 700 | 1 | |a Cao |D Ding-Chen |u Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China |4 aut | |
| 700 | 1 | |a Sun |D Xiao-Wen |u Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China |4 aut | |
| 773 | 0 | |t Aquaculture International |d Springer International Publishing |g 23/1(2015-02-01), 81-91 |x 0967-6120 |q 23:1<81 |1 2015 |2 23 |o 10499 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10499-014-9799-x |q text/html |z Onlinezugriff via DOI |
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| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10499-014-9799-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Ya-Juan |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Bo |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D He |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gao |D Yang-Chun |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gao |D Min |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Yu-Sheng |u Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cao |D Ding-Chen |u Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Xiao-Wen |u Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Aquaculture International |d Springer International Publishing |g 23/1(2015-02-01), 81-91 |x 0967-6120 |q 23:1<81 |1 2015 |2 23 |o 10499 | ||