caa a22 4500 605464146 CHVBK 20210128100256.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10499-014-9799-x doi (NATIONALLICENCE)springer-10.1007/s10499-014-9799-x Study of the genomic constitution of the German mirror carp [Elektronische Daten] [Ya-Juan Li, Bo Liu, He Zhou, Yang-Chun Gao, Min Gao, Yu-Sheng Wang, Ding-Chen Cao, Xiao-Wen Sun] Semen was treated with ultraviolet irradiation to genetically inactivate the sperm. Gynogenetic haploidy was induced artificially in the German mirror carp, Cyprinus carpio L. A systematic study of the status of early embryonic development, the number of chromosomes, chromosomal banding patterns and fluorescent in situ hybridization (FISH), and a microsatellite analysis were performed. The fertility, hatchability, and survival rates of the gynogenetic group were significantly lower than those of the normal control fertilization group (P<0.05). Gynogenetic larval growth was abnormal and the embryos did not survive. The chromosome number of the gynogenetic group was 50, whereas that of the control group was 100. The gynogenetic group had a single silver-staining point, one CMA3/DA/DAPI-positive position, and a single FISH-positive signal, whereas the control group had two silver-staining points, two CMA3/DA/DAPI-staining positions, and two FISH-positive signals. The microsatellite analysis showed that all the genes of the gynogenetic group derived from the female parent and that the females generated a set of haploid (n=50) gametes during meiosis. Using chromosome manipulation, this study confirms that the German mirror carp has two sets of chromosomes and is an evolutionary tetraploid (2n=100). Springer International Publishing Switzerland, 2014 Cyprinus carpio L nationallicence Gynogenetic nationallicence Ag-NORs nationallicence CMA3/DA/DAPI nationallicence Fluorescence in situ hybridization nationallicence Microsatellite analysis nationallicence A2 : Luteinizing hormone releasing hormone A2 for injection nationallicence CMA3 : Chromomysin A3 nationallicence DA : Distamycin A nationallicence DAPI : 4′6-Deamidino-2-phenylindole nationallicence DNA : Deoxyribonucleic acid nationallicence dNTPs : Deoxynucleotide triphosphates nationallicence 16-dUTP : Biotin-ε-aminocaproyl-γ-aminobutyryl-[5-(3-aminoallyl)-2′-deoxy-uridine-5′-triphate] tetralithium salt nationallicence DOM : Dopamine antagonist nationallicence FISH : Fluorescence in situ hybridization nationallicence MI : First meiotic division nationallicence NORs : Nucleolar organizer regions nationallicence PCR : Polymerase chain reaction nationallicence rDNA : Ribosomal deoxyribonucleic acid nationallicence UV : Ultraviolet nationallicence Li Ya-Juan Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut Liu Bo Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut Zhou He Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut Gao Yang-Chun Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut Gao Min Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut Wang Yu-Sheng Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut Cao Ding-Chen Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China aut Sun Xiao-Wen Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China aut Aquaculture International Springer International Publishing 23/1(2015-02-01), 81-91 0967-6120 23:1<81 2015 23 10499 https://doi.org/10.1007/s10499-014-9799-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9799-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Ya-Juan Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Liu Bo Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Zhou He Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Gao Yang-Chun Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Gao Min Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Wang Yu-Sheng Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, 116023, Dalian, China aut NATIONALLICENCE 700 1- Cao Ding-Chen Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China aut NATIONALLICENCE 700 1- Sun Xiao-Wen Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, 150070, Harbin, China aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/1(2015-02-01), 81-91 0967-6120 23:1<81 2015 23 10499