caa a22 4500 605464162 CHVBK 20210128100256.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10499-014-9813-3 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9813-3 Utilizing public aquariums and molecular identification techniques to address the larviculture potential of Pacific blue tangs ( Paracanthurus hepatus ), semicircle angelfish ( Pomacanthus semicirculatus ), and bannerfish ( Heniochus sp.) [Elektronische Daten] [Eric Cassiano, Matthew Wittenrich, Thomas Waltzek, Natalie Steckler, Kevin Barden, Craig Watson] Limitations in our understanding of aquaculture strategies necessary for cultivating many desirable species of marine ornamental fishes have impeded examining their commercial production. Currently, only a small fraction of species have been captive bred. Recent research efforts have focused on evaluating the rearing potential of marine fish eggs and larvae collected at public aquariums. However, as exhibits contain multiple species, accurate identification is crucial for determining species-specific larval rearing protocols. In this study, marine fish eggs and larvae were collected at four public display aquariums and were preserved for genetic identification prior to and during larviculture trials. During the project, numerous marine fish species were identified (n=14) and three desirable species displayed traits conducive to captive breeding. Pomacanthus semicirculatus was identified using molecular techniques prior to metamorphosis and verified morphologically once grown. This species was also reared through the larval phase and into the juvenile (or nursery) phase. However, two species (Paracanthurus hepatus and Heniochus sp.) did not reach metamorphosis and observations made during larviculture would not have been attributed to those particular species without the use of molecular identification techniques. Springer International Publishing Switzerland, 2014 DNA barcoding nationallicence Public aquarium nationallicence Pacific blue tang nationallicence Semicircle angelfish nationallicence Bannerfish nationallicence Cassiano Eric Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut Wittenrich Matthew Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut Waltzek Thomas Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, PO Box 110880, 32608, Gainesville, FL, USA aut Steckler Natalie Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, PO Box 110880, 32608, Gainesville, FL, USA aut Barden Kevin Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut Watson Craig Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut Aquaculture International Springer International Publishing 23/1(2015-02-01), 253-265 0967-6120 23:1<253 2015 23 10499 https://doi.org/10.1007/s10499-014-9813-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9813-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cassiano Eric Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut NATIONALLICENCE 700 1- Wittenrich Matthew Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut NATIONALLICENCE 700 1- Waltzek Thomas Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, PO Box 110880, 32608, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Steckler Natalie Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, PO Box 110880, 32608, Gainesville, FL, USA aut NATIONALLICENCE 700 1- Barden Kevin Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut NATIONALLICENCE 700 1- Watson Craig Tropical Aquaculture Laboratory, Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, University of Florida, 1408 24th Street S.E., 33570, Ruskin, FL, USA aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/1(2015-02-01), 253-265 0967-6120 23:1<253 2015 23 10499