caa a22 4500 605464227 CHVBK 20210128100257.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10499-014-9796-0 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9796-0 Comparison of temperature shock timing to induced artificial mitotic gynogenesis and androgenesis in common tench [Elektronische Daten] [Joanna Nowosad, Dariusz Kucharczyk, Tomasz Liszewski, Katarzyna Targońska, Roman Kujawa] The aim of the study was to determine the optimum temperature shock timing to induce androgenetic and gynogenetic growth in common tench (Tinca tinca L.). UV radiation at the dose of 3,456Jm−2 was used to inactivate the genome of oocytes (androgenesis) and sperm (gynogenesis). In each case, the optimum timing was sought (between 20 and 60min) before the beginning of diploidization of genetic material with a temperature shock (40°C, duration 2min). Eggs were incubated under laboratory conditions at 21°C. The highest survival rate for androgenotes was observed after the temperature shock was applied for 30min and for gynogenotes 40min after the activation. The survival rate for embryos did not exceed 2% in the best variants, either in andro- or gynogenesis. A negative correlation was found to exist between the quality of spawn and the occurrence of spontaneous andro- and gyno-genotes. The Author(s), 2014 Androgenesis nationallicence Gynogenesis nationallicence Spontaneous diploidization nationallicence Tench nationallicence UV gametes inactivation nationallicence Nowosad Joanna Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut Kucharczyk Dariusz Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut Liszewski Tomasz Department of Ichthyology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut Targońska Katarzyna Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut Kujawa Roman Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut Aquaculture International Springer International Publishing 23/1(2015-02-01), 45-53 0967-6120 23:1<45 2015 23 10499 https://doi.org/10.1007/s10499-014-9796-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9796-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nowosad Joanna Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut NATIONALLICENCE 700 1- Kucharczyk Dariusz Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut NATIONALLICENCE 700 1- Liszewski Tomasz Department of Ichthyology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut NATIONALLICENCE 700 1- Targońska Katarzyna Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut NATIONALLICENCE 700 1- Kujawa Roman Department of Lake and River Fisheries, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/1(2015-02-01), 45-53 0967-6120 23:1<45 2015 23 10499