caa a22 4500 605464359 CHVBK 20210128100257.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10499-015-9889-4 doi (NATIONALLICENCE)springer-10.1007/s10499-015-9889-4 Preliminary investigation into the effects of two dietary fatty acids, 20:5n-3 and 22:6n-3, on mortality of juvenile Mercenaria mercenaria during the approach to winter [Elektronische Daten] [Sixto Portilla, Brett Branco, John Tanacredi] Changes in the molar percentage of two omega-3 fatty acids, 20:5n-3 (EPA) and 22:6n-3 (DHA), occur in juvenile northern quahogs, Mercenaria mercenaria, as descending autumn temperatures approach winter lows in the temperate zone. Incorporation of these highly unsaturated fatty acids compensates for the effect of low temperature on cell membrane viscosity by lowering the phase transition of membrane phospholipids. Prior studies have demonstrated that the ability of bivalve mollusks, like M. mercenaria, to manufacture these compounds de novo does not exist or may be limited at best. Insufficient exogenous supply of EPA and DHA, therefore, may restrict their ability to overcome the inhibiting effect of high viscosity on cell membrane phospholipids during this interval. In the present study, a matrix of cultured algae containing various quantities of these compounds was administered to four groups of juvenile M. mercenaria as they adapted to low temperature. Each group displayed a unique response to diet in relative fatty acid profile and mortality. The group receiving abundant EPA adapted well during the early-phase temperature decline, and the one receiving abundant DHA adapted well to the late-phase temperature decline. The group receiving abundant DHA also enjoyed best overall survival following a severe 5-day drop in temperature. The strain native to the local region suffered between 31 and 117% higher mortality than the notata strain during the 72-day period of temperature decline from 18 to 6°C. Springer International Publishing Switzerland, 2015 Essential fatty acids nationallicence Homeoviscous adaptation nationallicence Low temperature acclimation nationallicence Mercenaria nationallicence Mortality nationallicence 20:5n-3 : Eicosapentaenoic acid nationallicence 22:6n-3 : Docosahexaenoic acid nationallicence 22:2 NMIs : 22-Carbon diene non-methylene-interrupted fatty acids nationallicence 16:0 : Palmitic acid nationallicence 18:3n-3 : [α] Alpha-linolenic acid nationallicence EPA : Eicosapentaenoic acid nationallicence DHA : Docosahexaenoic acid nationallicence ALA : [α] Alpha-linolenic acid nationallicence HUFA : Highly unsaturated fatty acid nationallicence HVA : Homeoviscous adaptation nationallicence MSL : Mean shell length nationallicence FA : Fatty acids nationallicence ANOVA : Analysis of variance nationallicence NOAA : National Oceanographic and Atmospheric Administration nationallicence FAME : Fatty acid methyl ester nationallicence GC : Gas chromatograph nationallicence ID : Inside diameter nationallicence Portilla Sixto Center for Environmental Research and Coastal Oceans Monitoring, Molloy College, 132 Clyde Street, 11796, West Sayville, NY, USA aut Branco Brett Aquatic Research and Environmental Assessment Center, Brooklyn College, 2900 Bedford Ave., 11210, Brooklyn, NY, USA aut Tanacredi John Center for Environmental Research and Coastal Oceans Monitoring, Molloy College, 132 Clyde Street, 11796, West Sayville, NY, USA aut Aquaculture International Springer International Publishing 23/6(2015-12-01), 1357-1376 0967-6120 23:6<1357 2015 23 10499 https://doi.org/10.1007/s10499-015-9889-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-015-9889-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Portilla Sixto Center for Environmental Research and Coastal Oceans Monitoring, Molloy College, 132 Clyde Street, 11796, West Sayville, NY, USA aut NATIONALLICENCE 700 1- Branco Brett Aquatic Research and Environmental Assessment Center, Brooklyn College, 2900 Bedford Ave., 11210, Brooklyn, NY, USA aut NATIONALLICENCE 700 1- Tanacredi John Center for Environmental Research and Coastal Oceans Monitoring, Molloy College, 132 Clyde Street, 11796, West Sayville, NY, USA aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/6(2015-12-01), 1357-1376 0967-6120 23:6<1357 2015 23 10499