caa a22 4500 605464383 CHVBK 20210128100257.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10499-015-9902-y doi (NATIONALLICENCE)springer-10.1007/s10499-015-9902-y Histological and micro-/macro-morphological evaluation of intestine in sharpsnout seabream ( Diplodus puntazzo ) fed soybean meal-based diets added with MOS and inulin as prebiotics [Elektronische Daten] [Ermete Ferrara, Andrea Gustinelli, Maria Fioravanti, Brunella Restucci, Francesco Quaglio, Stefania Marono, Giovanni Piccolo] The effects of mannanoligosaccharides (MOS) and inulin (INU) inclusion (8gkg−1) in a soybean meal (SBM)-containing diet on histology, macro- and micromorphology of proximal and distal intestine of sharpsnout seabream (Diplodus puntazzo) were evaluated. 144 sharpsnout seabream (100g average initial body weight) were fed with 4 isolipidic and isoproteic diets. At the end of the experimental period (114days), four fish per diet were randomly sampled. The partial substitution of fish meal (FM) with SBM affected the intestine histology and macromorphology of sharpsnout seabream and modified the enterocyte parameters. The addition of prebiotics has not produced positive effects, leading in some cases to a worsening, especially for INU diet that markedly altered the intestine histology. The micromorphological values were in both proximal and distal intestines significantly affected by the diet: in proximal tract, the nucleus was closer to the apex of the enterocyte in soy-fed fish (SBM, IMU, MOS diets), while fish fed with MOS diet showed the lowest value of enterocyte height, significantly different from the FM diet. In distal tract of intestine, fish fed with FM diet showed the highest value for all considered parameters, while MOS diet resulted in the lowest enterocyte height and supranucleus height among the four diets. SBM-containing diets (SBM, IMU, MOS) resulted in lower values than FM diet for the features of enterocyte nucleus. Microvilli height was not affected by the diet in both proximal and distal tracts. In conclusion in this study, the partial substitution of FM with SBM (40% on protein basis) induced inflammatory reaction of the gut in sharpsnout seabream, evidenced by histological analysis and changes in morphometric characters of villi and enterocytes, not amended by the addition of MOS and even worsened in the case of inulin. Springer International Publishing Switzerland, 2015 Mannanoligosaccharides nationallicence Inulin nationallicence Diplodus puntazzo nationallicence Soybean meal nationallicence Intestine histology nationallicence Intestine macromorphology nationallicence Intestine micromorphology nationallicence FM : Fish meal nationallicence SBM : Soybean meal nationallicence MOS : Mannanoligosaccharides nationallicence INU : Inulin nationallicence HE : Enterocyte height nationallicence hMV : Microvilli height nationallicence hSN : Supranucleus height nationallicence hN : Nucleus height nationallicence wN : Nucleus width nationallicence HF : Fold height nationallicence SPC : Soy protein concentrate nationallicence Ferrara Ermete Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut Gustinelli Andrea Department of Veterinary Medical Science, Alma Mater Studiorum University of Bologna, Bologna, Italy aut Fioravanti Maria Department of Veterinary Medical Science, Alma Mater Studiorum University of Bologna, Bologna, Italy aut Restucci Brunella Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut Quaglio Francesco Department of Comparative Biomedicine and Nutrition, University of Padova, Padova, Italy aut Marono Stefania Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut Piccolo Giovanni Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut Aquaculture International Springer International Publishing 23/6(2015-12-01), 1525-1537 0967-6120 23:6<1525 2015 23 10499 https://doi.org/10.1007/s10499-015-9902-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-015-9902-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferrara Ermete Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut NATIONALLICENCE 700 1- Gustinelli Andrea Department of Veterinary Medical Science, Alma Mater Studiorum University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Fioravanti Maria Department of Veterinary Medical Science, Alma Mater Studiorum University of Bologna, Bologna, Italy aut NATIONALLICENCE 700 1- Restucci Brunella Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut NATIONALLICENCE 700 1- Quaglio Francesco Department of Comparative Biomedicine and Nutrition, University of Padova, Padova, Italy aut NATIONALLICENCE 700 1- Marono Stefania Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut NATIONALLICENCE 700 1- Piccolo Giovanni Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Via F. Delpino n. 1, 80137, Napoli, Italy aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/6(2015-12-01), 1525-1537 0967-6120 23:6<1525 2015 23 10499