caa a22 4500 605464588 CHVBK 20210128100258.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10499-014-9851-x doi (NATIONALLICENCE)springer-10.1007/s10499-014-9851-x Protein replacement in practical diets altered gut allochthonous bacteria of cultured cyprinid species with different food habits [Elektronische Daten] [Zhimin Li, Li Xu, Wenshu Liu, Yuchun Liu, Einar Ringø, Zhenyu Du, Zhigang Zhou] As the cheap and sustainable recycled natural resources, intestinal casing meal (ICM) and yeast of monosodium glutamate (YMG) might be alternative protein sources in aquaculture diets. To determine the effects of dietary ICM and YMG on fish growth, feed utilization and gut allochthonous bacteria, 8-week feeding trials were conducted with blunt snoutbream (Megalobrama amblycephala, MA; a typical herbivorous cultured fish species in China), black carp (Mylopharyngodon piceus, MP; a typical carnivorous species in China) and gibel carp (Carassius gibelio, CG; a typical omnivorous species in China) with partial or complete replacement of soybean meal by ICM or ICM and YMG mixture. The results indicated that ICM and YMG incorporation had positive effects (P value >0.05) on weight gain and feed conversion rate. The complete replacement altered the gut allochthonous microbiota of gibel carp and black carp (Cs<0.70). Proteobacteria, Fusobacteria, Firmicutes and uncultured bacteria were predominant bacteria in these fish intestine by DGGE detection. Compared with the control diet group with SBM, intestinal allochthonous bacteria of fishes fed with ICM and YMG were significantly stimulated, such as proteobacterium clone (EF707282.1), Cetobacterium somerae (AB353124) and some Bacillus subtilis dependent on fish species. Based on these results, ICM and YMG have the potential to replace SBM in aqua feed, and altered gut allochthonous bacteria variedly as fish food habitats. Springer International Publishing Switzerland, 2014 Cyprinid fish nationallicence Food habit nationallicence Gut allochthonous microbiota nationallicence Protein replacement nationallicence CF : Condition factor nationallicence CG : Carassius gibelio nationallicence Cs : Coefficients nationallicence DGGE : 16S rDNA PCR-denaturing gradient gel electrophoresis nationallicence FBW : The final body weight of fish nationallicence FCR : Feed conversion ratio nationallicence IBW : The initial body weight of fish nationallicence ICM : Intestinal casing meal nationallicence ITd : The total dry diet consumption nationallicence MA : Megalobrama amblycephala nationallicence MP : Mylopharyngodon piceus nationallicence SBM : Soybean meal nationallicence SR : Survival rate nationallicence T : The experimental days nationallicence TI : Trypsin inhibitors nationallicence WG : Weight gains nationallicence YMG : Yeast of monosodium glutamate nationallicence Li Zhimin Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Xu Li Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Liu Wenshu Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Liu Yuchun Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Ringø Einar Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Du Zhenyu School of Life Sciences, East China Normal University, 200241, Shanghai, People's Republic of China aut Zhou Zhigang Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut Aquaculture International Springer International Publishing 23/4(2015-08-01), 913-928 0967-6120 23:4<913 2015 23 10499 https://doi.org/10.1007/s10499-014-9851-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9851-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Zhimin Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xu Li Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Wenshu Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yuchun Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Ringø Einar Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Du Zhenyu School of Life Sciences, East China Normal University, 200241, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Zhigang Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/4(2015-08-01), 913-928 0967-6120 23:4<913 2015 23 10499