caa a22 4500 60546460X CHVBK 20210128100258.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10499-014-9868-1 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9868-1 Low occurrence of WSSV in Penaeus monodon nauplii and post-larvae produced from PCR-negative broodstocks [Elektronische Daten] [Sabuj Mazumder, Mazlan Ghaffar, Simon Das, Md. Alam] White spot syndrome virus (WSSV) is a devastating, infectious virus affecting farming tiger shrimp (Penaeus monodon). In efforts to limit trans-boundary movement of this pathogen and reduce the economic and socioeconomic impact of disease in shrimp culture, there is considerable scope for more effective use of DNA-based methods of pathogen detection. To assess the prevalence of the WSSV, broods, nauplii and post-larvae (PL) samples were collected from nine shrimp hatcheries located in the southeast and southwest regions of Bangladesh during the period from February 2008 to May 2014 and subjected to screening for WSSV by polymerase chain reaction (PCR) technique. The prevalence of WSSV during the 7-year period ranged from 48.15 to 62.99%, 12.88 to 31.55% and 4.03 to 8.89% in broodstocks, nauplii and post-larvae, respectively. Out of a total of 5489 brood samples analyzed during the 7years, 2,977 were found to be PCR positive for WSSV giving an overall prevalence of 54.24%. The overall rates of PCR positive were 19.52% for nauplii and 6.25% for PL. The rates of infection in the nauplii and PL were much less than that observed in the broodstocks as the nauplii and PL samples were collected from broods found to be negative for WSSV by PCR. A follow-up study showed successful culture in about 87% of the shrimp farms stocked with the PL that were PCR negative for WSSV. This study confirmed that PCR is an effective diagnostic tool for the screening of broodstocks before being used for spawning in the hatchery, to prevent vertical transmission of WSSV from parents to larvae. However, to avail the advantage of PCR screening for a risk-free crop, sources of potential horizontal contaminations should also be controlled. Springer International Publishing Switzerland, 2014 Broodstock nationallicence Hatcheries nationallicence Penaeus monodon nationallicence White spot syndrome virus nationallicence PCR nationallicence DNA : Deoxyribonucleic acid nationallicence PCR : Polymerase chain reaction nationallicence WSSV : White spot syndrome virus nationallicence PL : Post-larvae nationallicence MT : Metric ton nationallicence Mazumder Sabuj Department of Aquatic Resource Management, Faculty of Fisheries, Sylhet Agricultural University, 3100, Sylhet, Bangladesh aut Ghaffar Mazlan Marine Ecosystem Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, DE, Malaysia aut Das Simon Marine Ecosystem Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, DE, Malaysia aut Alam Md Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, 2202, Mymensingh, Bangladesh aut Aquaculture International Springer International Publishing 23/4(2015-08-01), 1109-1123 0967-6120 23:4<1109 2015 23 10499 https://doi.org/10.1007/s10499-014-9868-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9868-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mazumder Sabuj Department of Aquatic Resource Management, Faculty of Fisheries, Sylhet Agricultural University, 3100, Sylhet, Bangladesh aut NATIONALLICENCE 700 1- Ghaffar Mazlan Marine Ecosystem Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, DE, Malaysia aut NATIONALLICENCE 700 1- Das Simon Marine Ecosystem Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, DE, Malaysia aut NATIONALLICENCE 700 1- Alam Md Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, 2202, Mymensingh, Bangladesh aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/4(2015-08-01), 1109-1123 0967-6120 23:4<1109 2015 23 10499