caa a22 4500 605464618 CHVBK 20210128100258.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10499-014-9865-4 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9865-4 Effect of plant proteins and crystalline amino acid supplementation on postprandial plasma amino acid profiles and metabolic response in rainbow trout ( Oncorhynchus mykiss ) [Elektronische Daten] [Marine Rolland, Bodil Larsen, Jørgen Holm, Johanne Dalsgaard, Peter Skov] The use of aquafeeds formulated with plant protein sources supplemented with crystalline amino acids (CAAs) is believed to influence amino acid (AA) uptake patterns and AA metabolic fate. Oxygen consumption and ammonia excretion rates were measured in rainbow trout (468.5±86.5g) force fed 0.75% of their body mass with a diet based on either (1) fish meal (FM), (2) pea protein concentrate (PPC), or (3) pea protein concentrate supplemented with histidine, lysine, methionine and threonine (PPC+) to mimic FM AA profile. The specific dynamic action and nitrogen quotient (NQ) were calculated for 48h of the postprandial period. In parallel, plasma AA concentrations were measured in blood samples withdrawn from the caudal vein before and then 2, 4, 6, 8, 12, 20, 32 and 48h after feed administration. The unbalanced diet PPC had a significantly higher NQ compared to FM (0.29±0.09 and 0.18±0.04, respectively), and plasma profiles of essential AAs reflected the dietary deficiencies. Supplementation with CAA in diet PPC+ resulted in an intermediary NQ (0.21±0.04) and significantly affected plasma AA profiles, presenting greater and faster rises followed by sharp decreases compared to FM. The strongest effect was observed for methionine, presenting threefold higher concentrations at peak time for PPC+ compared to FM (297.0±77.0 and 131.8±39.0nmolml−1, respectively). The differences in AA availability and metabolic profile in the pea diets compared to the FM diet were believed to be caused by an unbalanced dietary AA profile and CAA supplementation, rather than inclusion of plant protein concentrate. Springer International Publishing Switzerland, 2014 Essential amino acid nationallicence Non-essential amino acid nationallicence Ammonia excretion nationallicence Respirometry nationallicence SDA nationallicence AA : Amino acid nationallicence ACC : Acclimation nationallicence ANF : Anti-nutritional factor nationallicence CAA : Crystalline amino acid nationallicence DM : Dry matter nationallicence EAA : Essential amino acid nationallicence FM : Fish meal nationallicence NEAA : Non-essential amino acid nationallicence NQ : Nitrogen quotient nationallicence PI : Protein intake nationallicence PPC : Pea protein concentrate nationallicence SDA : Specific dynamic action nationallicence TAN : Total ammonia nitrogen nationallicence Rolland Marine BioMar A/S, Mylius Erichsensvej 35, 7330, Brande, Denmark aut Larsen Bodil DTU Aqua, Section for Aquaculture, The North Sea Research Center, Technical University of Denmark, P.O. Box 101, 9850, Hirtshals, Denmark aut Holm Jørgen BioMar A/S, Mylius Erichsensvej 35, 7330, Brande, Denmark aut Dalsgaard Johanne DTU Aqua, Section for Aquaculture, The North Sea Research Center, Technical University of Denmark, P.O. Box 101, 9850, Hirtshals, Denmark aut Skov Peter DTU Aqua, Section for Aquaculture, The North Sea Research Center, Technical University of Denmark, P.O. Box 101, 9850, Hirtshals, Denmark aut Aquaculture International Springer International Publishing 23/4(2015-08-01), 1071-1087 0967-6120 23:4<1071 2015 23 10499 https://doi.org/10.1007/s10499-014-9865-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9865-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rolland Marine BioMar A/S, Mylius Erichsensvej 35, 7330, Brande, Denmark aut NATIONALLICENCE 700 1- Larsen Bodil DTU Aqua, Section for Aquaculture, The North Sea Research Center, Technical University of Denmark, P.O. Box 101, 9850, Hirtshals, Denmark aut NATIONALLICENCE 700 1- Holm Jørgen BioMar A/S, Mylius Erichsensvej 35, 7330, Brande, Denmark aut NATIONALLICENCE 700 1- Dalsgaard Johanne DTU Aqua, Section for Aquaculture, The North Sea Research Center, Technical University of Denmark, P.O. Box 101, 9850, Hirtshals, Denmark aut NATIONALLICENCE 700 1- Skov Peter DTU Aqua, Section for Aquaculture, The North Sea Research Center, Technical University of Denmark, P.O. Box 101, 9850, Hirtshals, Denmark aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/4(2015-08-01), 1071-1087 0967-6120 23:4<1071 2015 23 10499