caa a22 4500 605464634 CHVBK 20210128100258.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10499-014-9862-7 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9862-7 Isolation and genotyping of potentially pathogenic Vibrio alginolyticus associated with Ruditapes decussatus larva and juvenile mass mortalities [Elektronische Daten] [Badreddine Mechri, Imen Ben Salem, Amel Medhioub, Mohamed Medhioub, Mahjoub Aouni] Recently, a series of mass mortalities of cultured Ruditapes decussatus have occurred in the bivalve hatchery of the National Institute of Marine Sciences and Technologies situated on the eastern littoral of Tunisia, North Africa. Vibrio alginolyticus species was predominantly isolated from dead and moribund veliger larva and juvenile. As far as we know, this is the first report of the involvement of this bacterium in clam epizootics in southern Mediterranean coastal areas. All bacterial isolates were biochemically identified, molecularly characterized by amplification of the Hsp-40 gene and analyzed by enterobacterial repetitive intergenic consensus-based PCR to evaluate genotypic relationships between the isolated strains. The antibiotics sensitivity, the lysozyme antibacterial effect and the in vitro cytotoxic activity (HeLa, Vero and Hep-2 cell lines) were also carried out. Furthermore, V. alginolyticus pathogenicity to R. decussatus larva was investigated using an experimental infection model. The majority of Vibrio isolates expressed high rates of antimicrobial resistance. The minimum inhibitory concentration results revealed that tetracycline and gentamycin were the most effective antibiotics against the tested strains. The phylogenetic analysis revealed relatedness between pathogenic isolates recovered from different locations. Vibrio cells were also able to resist to the bactericidal effect of the R. decussatus larva lysozyme. The extracellular products from V. alginolyticus isolates (L511, PL520 and N385) displayed significant cytotoxic effects against Vero and HeLa cells. The experimental challenge showed that all Vibrio strains exhibited high rates of mortality (up to 90%) in R. decussatus larva after 24-h exposure to high bacterial concentration (107ufcmL−1). Springer International Publishing Switzerland, 2014 Ruditapes decussatus nationallicence Vibrio alginolyticus nationallicence Hatchery nationallicence Antibiotics nationallicence ERIC nationallicence Experimental infection nationallicence Mechri Badreddine Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisie aut Ben Salem Imen Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisie aut Medhioub Amel Laboratoire d'Aquaculture, Institut National des Sciences et Technologies de la Mer, Route de Khniss, B.P. 59, 5000, Monastir, Tunisie aut Medhioub Mohamed Laboratoire d'Aquaculture, Institut National des Sciences et Technologies de la Mer, Route de Khniss, B.P. 59, 5000, Monastir, Tunisie aut Aouni Mahjoub Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisie aut Aquaculture International Springer International Publishing 23/4(2015-08-01), 1033-1047 0967-6120 23:4<1033 2015 23 10499 https://doi.org/10.1007/s10499-014-9862-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9862-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mechri Badreddine Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisie aut NATIONALLICENCE 700 1- Ben Salem Imen Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisie aut NATIONALLICENCE 700 1- Medhioub Amel Laboratoire d'Aquaculture, Institut National des Sciences et Technologies de la Mer, Route de Khniss, B.P. 59, 5000, Monastir, Tunisie aut NATIONALLICENCE 700 1- Medhioub Mohamed Laboratoire d'Aquaculture, Institut National des Sciences et Technologies de la Mer, Route de Khniss, B.P. 59, 5000, Monastir, Tunisie aut NATIONALLICENCE 700 1- Aouni Mahjoub Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculté de Pharmacie, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisie aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/4(2015-08-01), 1033-1047 0967-6120 23:4<1033 2015 23 10499