caa a22 4500 605464677 CHVBK 20210128100259.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10499-014-9863-6 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9863-6 Metabolic, immune responses in prawn ( Penaeus monodon ) exposed to ambient ammonia [Elektronische Daten] [Lishi Yang, Qibin Yang, Shigui Jiang, Yong Li, Falin Zhou, Tao Li, Jianhua Huang] This article reports on the changes in the metabolic detoxification indices of the hepatopancreas, muscle, and gill tissues of juvenile prawn (body length of 5.0±0.5cm) of Penaeus monodon at different ammonia levels (at concentrations of 0, 10, 20, and 30mg/L) as well as the immune-related gene expressions of the prawn under elevated ammonia (30mg/L) stress. Results showed that glutamine synthetase (GS) activity and glutamine (Gln) content were higher in the muscle than those in the gill and hepatopancreas, not only at the normal level but also under stress. The GS activity and the Gln concentration at 96h were significantly higher than those in the control group (p<0.05) and positively correlated with the ammonia concentration from 0 to 48h. The gene expressions of C-lysozyme, antibacterial peptide (crustin), and antilipopolysaccharide factor in P. monodon were upregulated at the initial period and downregulated at the later period under high ammonia stress (30mg/L ammonia), except for the crustin gene in muscle, which maintained a continuous high expression from 0 to 96h (p<0.05). Our results confirmed that ammonia stress induced a detoxification metabolic pathway at least dependent on Gln synthesis in juvenile prawn. Body immunity was also activated at the initial period and decreased at the later period of high ammonia stress. Muscle is a sensitive tissue reflecting the ammonia stress state not only by physiological index but also by crustin mRNA level in juvenile prawn. Springer International Publishing Switzerland, 2014 Penaeus monodon nationallicence Juvenile nationallicence Ammonia toxicity nationallicence Metabolic detoxification nationallicence Innate immunity nationallicence Yang Lishi Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut Yang Qibin Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut Jiang Shigui Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut Li Yong Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut Zhou Falin Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut Li Tao Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut Huang Jianhua Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut Aquaculture International Springer International Publishing 23/4(2015-08-01), 1049-1062 0967-6120 23:4<1049 2015 23 10499 https://doi.org/10.1007/s10499-014-9863-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9863-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Lishi Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Qibin Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Shigui Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Yong Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Falin Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Tao Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Huang Jianhua Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/4(2015-08-01), 1049-1062 0967-6120 23:4<1049 2015 23 10499