caa a22 4500 605464715 CHVBK 20210128100259.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10499-014-9847-6 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9847-6 Hepatoprotective and antioxidant effects of phyllanthin against carbon tetrachloride-induced liver injury in Cyprinus carpio [Elektronische Daten] [Rui Jia, Jin-Liang Du, Li-Ping Cao, Ying-Juan Liu, Pao Xu, Guo-Jun Yin] The aim of the present study was to investigate the protective effects and possible mechanism of phyllanthin against carbon tetrachloride (CCl4)-induced hepatic damage in common carp. Fish were fed diet containing four doses of phyllanthin (0, 100, 300, and 600mg/kg diet) for 60days, and then given an intraperitoneal injection of CCl4 (30% in arachis oil) at a dose of 0.5ml/kg body weight. At 72h after CCl4 injection, blood and liver samples were collected for the analyses of serum biochemical parameters, peroxidation product, glutathione, and antioxidant enzyme activities. The results showed that administration of phyllanthin at the doses of 300 and 600mg/kg in the diet prior to CCl4 intoxication considerably inhibited the adverse alterations caused by CCl4 in serum and liver tissue, including glutamate pyruvate transaminase, glutamate oxalate transaminase, lactate dehydrogenase, total protein, albumin, superoxide dismutase, glutathione peroxidase, catalase, glutathione, total antioxidant capacity and malondialdehyde. Furthermore, the positive effects of phyllanthin were confirmed in histopathologic examination. Overall results proved the hepatoprotection of phyllanthin, and it is suggested that phyllanthin may be used as a hepatoprotective agent to prevent liver diseases in fish. Springer International Publishing Switzerland, 2014 Phyllanthin nationallicence Carbon tetrachloride nationallicence Hepatoprotective effect nationallicence Cyprinus carpio nationallicence Jia Rui Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut Du Jin-Liang Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut Cao Li-Ping Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut Liu Ying-Juan Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, 214081, Wuxi, China aut Xu Pao Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut Yin Guo-Jun Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut Aquaculture International Springer International Publishing 23/4(2015-08-01), 883-893 0967-6120 23:4<883 2015 23 10499 https://doi.org/10.1007/s10499-014-9847-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9847-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jia Rui Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Du Jin-Liang Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Cao Li-Ping Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Liu Ying-Juan Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, 214081, Wuxi, China aut NATIONALLICENCE 700 1- Xu Pao Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Yin Guo-Jun Wuxi Fisheries College, Nanjing Agricultural University, 214081, Wuxi, Jiangsu, China aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/4(2015-08-01), 883-893 0967-6120 23:4<883 2015 23 10499