caa a22 4500 605464774 CHVBK 20210128100259.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10499-014-9871-6 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9871-6 The carbohydrate metabolism of scallop Chlamys farreri in the immune response against acute challenge of Vibrio anguillarum [Elektronische Daten] [Xingqiang Wang, Lingling Wang, Huan Zhang, Rui Liu, Linsheng Song] Carbohydrate metabolism may play an important role in maintaining cell homeostasis in bivalves. In the present study, the temporal variations in immune and carbohydrate metabolism parameters of Zhikong scallop Chlamys farreri under an acute Vibrio anguillarum challenge were investigated in order to better understand the energetic mechanisms of scallop immune defense. After bacterial challenge, reactive oxygen species, total antioxidant capacity, acid phosphatase activity, and transcripts of peptidoglycan recognition protein and heat-shock protein 70 in the hemolymph of scallops increased substantially within 96h. By contrast, the significantly increased malondialdehyde content was observed in the serum of challenged scallops. As far as carbohydrate metabolism was concerned, the expression levels of hexokinase, isocitrate dehydrogenase, and N-acetylglucosaminyltransferase VI in hemocytes of challenged scallops were significantly up-regulated within 192h. At 192h, the electron transport system budgets were significantly higher, while the glycogen contents were significantly lower in soft tissues of challenged scallops as compared to those of control. Moreover, the significantly increased glucose content concomitant with significantly up-regulated expressions of sodium glucose transporter 1 and glucose-regulated protein 78 was observed in the hemolymph at 24h after challenge. The above results demonstrated that bacterial challenge could induce acute immune responses and cause oxidative stress in scallops. During the bacterial stress period, scallops utilized glucose via anaerobic glycolysis and aerobic oxidation to meet the overall cost of immune response. Also, scallops might rely upon the metabolic pathways of glycogen degradation and gluconeogenesis to maintain glucose homeostasis in hemolymph. Springer International Publishing Switzerland, 2014 Vibrio anguillarum nationallicence Glucose metabolism nationallicence Glucose transport nationallicence Glycogenolysis nationallicence Immune response nationallicence Zhikong scallop nationallicence Wang Xingqiang Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Wang Lingling Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Zhang Huan Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Liu Rui Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Song Linsheng Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut Aquaculture International Springer International Publishing 23/5(2015-10-01), 1141-1155 0967-6120 23:5<1141 2015 23 10499 https://doi.org/10.1007/s10499-014-9871-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9871-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Xingqiang Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Wang Lingling Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Zhang Huan Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Liu Rui Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 700 1- Song Linsheng Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/5(2015-10-01), 1141-1155 0967-6120 23:5<1141 2015 23 10499