caa a22 4500 605464839 CHVBK 20210128100259.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10499-014-9870-7 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9870-7 Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5% feed supplementation with F. benghalensis (aerial root) and L. leucocephala (pod seed) [Elektronische Daten] [Vipin Verma, Kumari Rani, Neeta Sehgal, Om Prakash] Ficus benghalensis and Leucaena leucocephala are perennial trees commonly known for their wide medicinal use in India. Immuno-stimulatory effect of F. benghalensis (aerial root) or L. leucocephala (pod seeds) 5% powder as supplementary ingredients in the artificial fish feed of Indian spotted snakehead, Channa punctata, was compared with control group (fish fed with non-supplemented artificial feed). Fish were challenged with Aeromonas hydrophila, a major disease causing bacteria in freshwater fish on weekly intervals for four consecutive weeks. Blood and tissue samples were collected on 7th, 14th, 21st and 28thday after challenge. The specific immunity (antibody response) and non-specific immunity (lysozyme activity, phagocytic response, nitric oxide and SOD levels) of the fish were evaluated. The increase in the levels of SGOT and SGPT in liver of fish fed with non-supplemented feed indicated damage of most targeting organs by A. hydrophila. However, the levels did not changed significantly in fish fed with experimental feed till the end of experiment. Higher levels of nitric oxide and superoxide dismutase indicated lower stress in fish fed with supplementary feed compared to control group. Fish fed with supplementary feed also showed increase in lysozyme and phagocytic activity. The levels of immunoglobulin in the serum were determined by sandwich ELISA. Higher antibody production in fish fed with supplementary feeds indicated enhanced immune response. The present study highlights the potential immuno-stimulatory response of powder of F. benghalensis and L. leucocephala when supplemented in the artificial feed of C. punctata. Springer International Publishing Switzerland, 2014 Supplementary feed nationallicence Innate/humoral immunity nationallicence Ficus benghalensis nationallicence Leucaena leucocephala nationallicence Channa punctata nationallicence Verma Vipin Department of Zoology, Sri Venkateswara College, University of Delhi, Dhaula Kuan, 110021, New Delhi, India aut Rani Kumari Department of Zoology, Kalindi College, University of Delhi, Dhaula Kuan, East Patel Nagar, 110008, New Delhi, India aut Sehgal Neeta Department of Zoology, University of Delhi, 110007, Delhi, India aut Prakash Om Department of Zoology, Sri Venkateswara College, University of Delhi, Dhaula Kuan, 110021, New Delhi, India aut Aquaculture International Springer International Publishing 23/5(2015-10-01), 1127-1140 0967-6120 23:5<1127 2015 23 10499 https://doi.org/10.1007/s10499-014-9870-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9870-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verma Vipin Department of Zoology, Sri Venkateswara College, University of Delhi, Dhaula Kuan, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Rani Kumari Department of Zoology, Kalindi College, University of Delhi, Dhaula Kuan, East Patel Nagar, 110008, New Delhi, India aut NATIONALLICENCE 700 1- Sehgal Neeta Department of Zoology, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Prakash Om Department of Zoology, Sri Venkateswara College, University of Delhi, Dhaula Kuan, 110021, New Delhi, India aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/5(2015-10-01), 1127-1140 0967-6120 23:5<1127 2015 23 10499