caa a22 4500 605464855 CHVBK 20210128100300.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10499-014-9874-3 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9874-3 LAMP assay to detect Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in shrimp [Elektronische Daten] [Jetnaphang Kongrueng, Natta Tansila, Pimonsri Mitraparp-arthorn, Mitsuaki Nishibuchi, Gary Vora, Varaporn Vuddhakul] Acute hepatopancreatic necrosis disease (AHPND) is a serious disease in shrimp and results in considerable losses for the shrimp aquaculture industry. The etiologic agent of AHPND has recently been identified as a unique strain of Vibrio parahaemolyticus: One that is different from human pathogenic V. parahaemolyticus strains. In this study, two sets of primers (LAMP-A2 and LAMP-A3) were developed and validated for use in a LAMP assay to specifically identify V. parahaemolyticus causing AHPND (V. parahaemolyticus AHPND). LAMP-A2 and LAMP-A3 detected all 33 V. parahaemolyticus AHPND isolates except the non-V. parahaemolyticus AHPND isolates and 19 other closely related bacterial species. In pure culture and in spiked shrimp experiments, the LAMP assay was superior to PCR for the detection of V. parahaemolyticus AHPND. In pure cultures, the detection limit of LAMP-A3 was 53CFU/ml or 0.1CFU per reaction (10× lower than LAMP-A2), whereas in spiked shrimp experiments, the detection limit was 4.4×105CFU/ml or 8.8×102 CFU per reaction. Further testing of 24 post-larvae, shrimp, sediment and water samples collected from a shrimp farm revealed that V. parahaemolyticus AHPND was detected mostly in the sediment samples. Taken together, the results suggested that the LAMP-A3-based LAMP assay, but not LAMP-A2 or PCR, was suitable for the identification of V. parahaemolyticus AHPND in shrimp aquaculture. Springer International Publishing Switzerland, 2015 Vibrio parahaemolyticus nationallicence AHPND nationallicence Shrimp nationallicence LAMP nationallicence Vibriosis nationallicence Kongrueng Jetnaphang Food safety and health research unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand aut Tansila Natta Faculty of Medical Technology, Prince of Songkla University, Songkhla, Thailand aut Mitraparp-arthorn Pimonsri Food safety and health research unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand aut Nishibuchi Mitsuaki Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan aut Vora Gary Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA aut Vuddhakul Varaporn Food safety and health research unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand aut Aquaculture International Springer International Publishing 23/5(2015-10-01), 1179-1188 0967-6120 23:5<1179 2015 23 10499 https://doi.org/10.1007/s10499-014-9874-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9874-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kongrueng Jetnaphang Food safety and health research unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand aut NATIONALLICENCE 700 1- Tansila Natta Faculty of Medical Technology, Prince of Songkla University, Songkhla, Thailand aut NATIONALLICENCE 700 1- Mitraparp-arthorn Pimonsri Food safety and health research unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand aut NATIONALLICENCE 700 1- Nishibuchi Mitsuaki Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan aut NATIONALLICENCE 700 1- Vora Gary Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA aut NATIONALLICENCE 700 1- Vuddhakul Varaporn Food safety and health research unit, Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/5(2015-10-01), 1179-1188 0967-6120 23:5<1179 2015 23 10499