caa a22 4500 605464863 CHVBK 20210128100300.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10499-015-9883-x doi (NATIONALLICENCE)springer-10.1007/s10499-015-9883-x Effect of dietary supplementation of Bacillus subtilis on haematological and immunological parameters of Catla catla (Hamilton) [Elektronische Daten] [Rajesh Kumar, Subhas Mukherjee, Ritesh Ranjan, T. Vani, Rajeev Brahmachari, Sukanta Nayak] Effect of dietary supplementation of a Gram-positive, aerobic, probiotic bacterium Bacillus subtilis on the immunohaematological indices during pre- and post-challenge in Indian major carp, catla (Catla catla), was studied. The B. subtilis was administered orally at four different doses 1.0×106 (T1), 1.0×107 (T2), 1.0×108 (T3), and 1.0×109 (T4) cfug−1 feed to C. catla for 90days. The positive control (Cp) and negative control (Cn) were fed with feed without B. subtilis for the same period. On the 60thday, blood and serum were sampled to determine various haematological and serum parameters. Fish were challenged intraperitoneally with Aeromonas hydrophila after 60days in all the treatment groups and Cp, while the Cn was challenged with phosphate-buffered saline (PBS, pH 7.2) only. Dietary supplementation of B. subtilis leads to the rise of various immunological and haematological parameters in catla during the pre- and post-challenge. During pre-challenge, the highest TEC (1.30±0.02×106cells mm−3), haemoglobin (7.43±0.25g%), total serum protein (3.89±0.08gdL−1), and serum lysozyme activity (8.39±0.01µgml−1) were recorded in fishes fed feed containing B. subtilis at 1×109 cfu/g feed (T4). The highest survival percentage (86.33%) was also observed in T4 group. The significantly increased survival percentage (P<0.05) of B. subtilis-treated groups in comparison with control group (Cp) suggests that dietary supplementation of this probiotic bacterium can protect catla from A. hydrophila infection by enhancing innate immunity. Springer International Publishing Switzerland, 2015 Bacillus subtilis nationallicence Aeromonas hydrophila nationallicence Catla catla nationallicence Immunity nationallicence Respiratory burst activity nationallicence Total erythrocyte count nationallicence Kumar Rajesh Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut Mukherjee Subhas Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut Ranjan Ritesh Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut Vani T. Inland Aquaculture Division, CIFE, 400 061, Mumbai, India aut Brahmachari Rajeev Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut Nayak Sukanta Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut Aquaculture International Springer International Publishing 23/5(2015-10-01), 1275-1292 0967-6120 23:5<1275 2015 23 10499 https://doi.org/10.1007/s10499-015-9883-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-015-9883-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumar Rajesh Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut NATIONALLICENCE 700 1- Mukherjee Subhas Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut NATIONALLICENCE 700 1- Ranjan Ritesh Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut NATIONALLICENCE 700 1- Vani T. Inland Aquaculture Division, CIFE, 400 061, Mumbai, India aut NATIONALLICENCE 700 1- Brahmachari Rajeev Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut NATIONALLICENCE 700 1- Nayak Sukanta Aquatic Animal Health Management Division, Central Institute of Fisheries Education (CIFE), 400 061, Mumbai, India aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/5(2015-10-01), 1275-1292 0967-6120 23:5<1275 2015 23 10499