Experimental infection of infectious myonecrosis virus (IMNV) in the Pacific white shrimp Litopenaeus vannamei (Boone, 1931)
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| 024 | 7 | 0 | |a 10.1007/s10499-014-9836-9 |2 doi |
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| 245 | 0 | 0 | |a Experimental infection of infectious myonecrosis virus (IMNV) in the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) |h [Elektronische Daten] |c [Suzianny da Silva, João Rocha, Pedro Martins, Alfredo Gálvez, Fernando dos Santos, Humber Andrade, Maria Coimbra] |
| 520 | 3 | |a Bioassays are essential in detecting resistant animals based on standardized challenge tests. A controlled bioassay system for Infectious myonecrosis virus (IMNV) was developed through two methods of infection, by ingestion and injection, in Litopenaeus vannamei. For ingestion bioassays, shrimp (2-3.9g) were exposed to four feeding infection treatments (feeding with infected tissue-FIT without stress; FITS-salinity stress; FITES-temperature and salinity stresses and FITA-alkalinity stress). No signs of disease were observed and 30% of the animals were infected, with FITS treatment exhibited the higher proportion of infected animals (p≤0.05). In the injection treatments, a stock inoculum was quantified (viral load of 9.82×105 IMNV copiesµg−1 of total RNA) and a tenfold serial dilution was tested at 28°C and, from 10−5 to 10−9 at 26°C. Over 60% of infected animals were obtained for dilutions between 10−5 and 10−7 at 28°C, while at 26°C; these values ranged from 30 to 50%, with a clear effect of low water temperature in reducing the proportion of infected shrimp. In general, higher survival probabilities of infected shrimp were achieved 5days after exposure, followed by a quick decrease at the tenth day post-inoculation. Median infectious dose (ID50) was determined between 2.44×10−8 and 1.19×10−7 at 28°C and between 1.76×10−6 and 1.28×10−5 at 26°C for 15days post-infection, respectively. Injection treatments based on known infectious dose were the most reliable method of estimating viral infectivity for IMNV selection. Moreover, FITS treatment was identified as an environmental risk factor, contributing to increase in IMNV infection prevalence. | |
| 540 | |a Springer International Publishing Switzerland, 2014 | ||
| 690 | 7 | |a Breeding program |2 nationallicence | |
| 690 | 7 | |a ID50 |2 nationallicence | |
| 690 | 7 | |a IMNV resistant shrimp |2 nationallicence | |
| 690 | 7 | |a Survival |2 nationallicence | |
| 690 | 7 | |a Virus infection |2 nationallicence | |
| 690 | 7 | |a IMNV : Infectious myonecrosis virus |2 nationallicence | |
| 690 | 7 | |a TSV : Taura syndrome virus |2 nationallicence | |
| 690 | 7 | |a WSSV : White spot syndrome virus |2 nationallicence | |
| 690 | 7 | |a SPF : Specific pathogen free |2 nationallicence | |
| 690 | 7 | |a OIE : World Organisation for Animal Health |2 nationallicence | |
| 690 | 7 | |a IHHNV : Infectious hypodermal and hematopoietic necrosis virus |2 nationallicence | |
| 690 | 7 | |a NHPB : Necrotizing hepatopancreatic bacterium |2 nationallicence | |
| 690 | 7 | |a PCR : Polymerase chain reaction |2 nationallicence | |
| 690 | 7 | |a TN buffer : Tris/NaCl buffer |2 nationallicence | |
| 690 | 7 | |a FITS : Feeding with infected tissue with salinity stress |2 nationallicence | |
| 690 | 7 | |a FITES : Feeding with infected tissue with temperature and salinity stresses |2 nationallicence | |
| 690 | 7 | |a FITA : Feeding with infected tissue with alkalinity stress |2 nationallicence | |
| 690 | 7 | |a FIT : Feeding with infected tissue without stress |2 nationallicence | |
| 690 | 7 | |a RT-PCR : Reverse transcription polymerase chain reaction |2 nationallicence | |
| 690 | 7 | |a GLM : Generalized linear models |2 nationallicence | |
| 690 | 7 | |a H&E : Hematoxylin and eosin stain |2 nationallicence | |
| 690 | 7 | |a ID50 : Median infectious dose |2 nationallicence | |
| 700 | 1 | |a da Silva |D Suzianny |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | |
| 700 | 1 | |a Rocha |D João |u Genearch Aquacultura Ltda, Rua Pedro Zuca, 59578-000, Rio do Fogo, Rio Grande do Norte, Brazil |4 aut | |
| 700 | 1 | |a Martins |D Pedro |u Departamento de Oceanografia e Limnologia, Universidade Federal do Rio Grande do Norte, Via Costeira, 59014-100, Praia de Mãe Luiza, Rio Grande do Norte, Brazil |4 aut | |
| 700 | 1 | |a Gálvez |D Alfredo |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | |
| 700 | 1 | |a dos Santos |D Fernando |u Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | |
| 700 | 1 | |a Andrade |D Humber |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | |
| 700 | 1 | |a Coimbra |D Maria |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | |
| 773 | 0 | |t Aquaculture International |d Springer International Publishing |g 23/2(2015-04-01), 563-576 |x 0967-6120 |q 23:2<563 |1 2015 |2 23 |o 10499 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10499-014-9836-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10499-014-9836-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a da Silva |D Suzianny |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rocha |D João |u Genearch Aquacultura Ltda, Rua Pedro Zuca, 59578-000, Rio do Fogo, Rio Grande do Norte, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Martins |D Pedro |u Departamento de Oceanografia e Limnologia, Universidade Federal do Rio Grande do Norte, Via Costeira, 59014-100, Praia de Mãe Luiza, Rio Grande do Norte, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gálvez |D Alfredo |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a dos Santos |D Fernando |u Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Andrade |D Humber |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Coimbra |D Maria |u Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Aquaculture International |d Springer International Publishing |g 23/2(2015-04-01), 563-576 |x 0967-6120 |q 23:2<563 |1 2015 |2 23 |o 10499 | ||