caa a22 4500 605464944 CHVBK 20210128100300.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10499-014-9836-9 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9836-9 Experimental infection of infectious myonecrosis virus (IMNV) in the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) [Elektronische Daten] [Suzianny da Silva, João Rocha, Pedro Martins, Alfredo Gálvez, Fernando dos Santos, Humber Andrade, Maria Coimbra] Bioassays are essential in detecting resistant animals based on standardized challenge tests. A controlled bioassay system for Infectious myonecrosis virus (IMNV) was developed through two methods of infection, by ingestion and injection, in Litopenaeus vannamei. For ingestion bioassays, shrimp (2-3.9g) were exposed to four feeding infection treatments (feeding with infected tissue-FIT without stress; FITS-salinity stress; FITES-temperature and salinity stresses and FITA-alkalinity stress). No signs of disease were observed and 30% of the animals were infected, with FITS treatment exhibited the higher proportion of infected animals (p≤0.05). In the injection treatments, a stock inoculum was quantified (viral load of 9.82×105 IMNV copiesµg−1 of total RNA) and a tenfold serial dilution was tested at 28°C and, from 10−5 to 10−9 at 26°C. Over 60% of infected animals were obtained for dilutions between 10−5 and 10−7 at 28°C, while at 26°C; these values ranged from 30 to 50%, with a clear effect of low water temperature in reducing the proportion of infected shrimp. In general, higher survival probabilities of infected shrimp were achieved 5days after exposure, followed by a quick decrease at the tenth day post-inoculation. Median infectious dose (ID50) was determined between 2.44×10−8 and 1.19×10−7 at 28°C and between 1.76×10−6 and 1.28×10−5 at 26°C for 15days post-infection, respectively. Injection treatments based on known infectious dose were the most reliable method of estimating viral infectivity for IMNV selection. Moreover, FITS treatment was identified as an environmental risk factor, contributing to increase in IMNV infection prevalence. Springer International Publishing Switzerland, 2014 Breeding program nationallicence ID50 nationallicence IMNV resistant shrimp nationallicence Survival nationallicence Virus infection nationallicence IMNV : Infectious myonecrosis virus nationallicence TSV : Taura syndrome virus nationallicence WSSV : White spot syndrome virus nationallicence SPF : Specific pathogen free nationallicence OIE : World Organisation for Animal Health nationallicence IHHNV : Infectious hypodermal and hematopoietic necrosis virus nationallicence NHPB : Necrotizing hepatopancreatic bacterium nationallicence PCR : Polymerase chain reaction nationallicence TN buffer : Tris/NaCl buffer nationallicence FITS : Feeding with infected tissue with salinity stress nationallicence FITES : Feeding with infected tissue with temperature and salinity stresses nationallicence FITA : Feeding with infected tissue with alkalinity stress nationallicence FIT : Feeding with infected tissue without stress nationallicence RT-PCR : Reverse transcription polymerase chain reaction nationallicence GLM : Generalized linear models nationallicence H&E : Hematoxylin and eosin stain nationallicence ID50 : Median infectious dose nationallicence da Silva Suzianny Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut Rocha João Genearch Aquacultura Ltda, Rua Pedro Zuca, 59578-000, Rio do Fogo, Rio Grande do Norte, Brazil aut Martins Pedro Departamento de Oceanografia e Limnologia, Universidade Federal do Rio Grande do Norte, Via Costeira, 59014-100, Praia de Mãe Luiza, Rio Grande do Norte, Brazil aut Gálvez Alfredo Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut dos Santos Fernando Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut Andrade Humber Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut Coimbra Maria Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut Aquaculture International Springer International Publishing 23/2(2015-04-01), 563-576 0967-6120 23:2<563 2015 23 10499 https://doi.org/10.1007/s10499-014-9836-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9836-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- da Silva Suzianny Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- Rocha João Genearch Aquacultura Ltda, Rua Pedro Zuca, 59578-000, Rio do Fogo, Rio Grande do Norte, Brazil aut NATIONALLICENCE 700 1- Martins Pedro Departamento de Oceanografia e Limnologia, Universidade Federal do Rio Grande do Norte, Via Costeira, 59014-100, Praia de Mãe Luiza, Rio Grande do Norte, Brazil aut NATIONALLICENCE 700 1- Gálvez Alfredo Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- dos Santos Fernando Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- Andrade Humber Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut NATIONALLICENCE 700 1- Coimbra Maria Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/2(2015-04-01), 563-576 0967-6120 23:2<563 2015 23 10499