Comparison of oral, rectal and intraperitoneal administration of IgY antibodies in passive immunization of rainbow trout ( Oncorhynchus mykiss )
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| 024 | 7 | 0 | |a 10.1007/s10499-014-9823-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10499-014-9823-1 | ||
| 245 | 0 | 0 | |a Comparison of oral, rectal and intraperitoneal administration of IgY antibodies in passive immunization of rainbow trout ( Oncorhynchus mykiss ) |h [Elektronische Daten] |c [Anja Winkelbach, Rüdiger Schade, Carsten Schulz, Sven Wuertz] |
| 520 | 3 | |a IgY antibodies serve an interesting opportunity for drug administration and passive immunization. For routine application in aquaculture, oral delivery needs to be established. Two feeding trials with non-specific IgY antibodies were conducted in juvenile trout to assess IgY uptake through the intestinal wall and to identify target tissue of IgY. In experiment 1, a positive control that received the lowest effective dose by intraperitoneal injection (0.0076mg IgY gBW−1day−1) was compared with three groups fed a 10-, 50- and 75-fold dosage and a negative control. After 12, 24 and 36h, plasma IgY concentrations of all treatments were below the detection threshold. In experiment 2, an excessive oral administration (200-fold) was compared with two groups that received IgY rectally at 75- and 150-fold dosage of the injected control. Blood samples taken 2, 4, 6, 12 and 24h did not result in detectable IgY concentrations, suggesting that neither the increased oral dosage nor the bypassing of the stomach improved IgY uptake. In both experiments, only intraperitoneal injection resulted in IgY uptake, revealing a decrease from 40.33±21.72µgml−1 after 12h to 19.48±12.67µgml−1 at 36h in experiment 1 and a peak at 75.58±23.75µgml−1 after 2h in experiment 2. Immunohistochemistry was carried out to localize IgY in selected target tissues of IgY action. Staining revealed IgY in the liver but not in white muscle, pituitary and brain, irrespective from the time point measured. | |
| 540 | |a Springer International Publishing Switzerland, 2014 | ||
| 690 | 7 | |a Growth |2 nationallicence | |
| 690 | 7 | |a IgY antibody |2 nationallicence | |
| 690 | 7 | |a Immunohistochemistry |2 nationallicence | |
| 690 | 7 | |a Passive immunization |2 nationallicence | |
| 690 | 7 | |a Oral vaccination |2 nationallicence | |
| 690 | 7 | |a Rainbow trout |2 nationallicence | |
| 700 | 1 | |a Winkelbach |D Anja |u Gesellschaft für Marine Aquakultur mbH, Hafentörn 3, 25761, Büsum, Germany |4 aut | |
| 700 | 1 | |a Schade |D Rüdiger |u Institute of Virology, Charité-Universitätsmedizin Berlin, 10117, Berlin, Germany |4 aut | |
| 700 | 1 | |a Schulz |D Carsten |u Gesellschaft für Marine Aquakultur mbH, Hafentörn 3, 25761, Büsum, Germany |4 aut | |
| 700 | 1 | |a Wuertz |D Sven |u Department of Aquaculture and Ecophysiology, Leibnitz Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587, Berlin, Germany |4 aut | |
| 773 | 0 | |t Aquaculture International |d Springer International Publishing |g 23/2(2015-04-01), 427-438 |x 0967-6120 |q 23:2<427 |1 2015 |2 23 |o 10499 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10499-014-9823-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10499-014-9823-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Winkelbach |D Anja |u Gesellschaft für Marine Aquakultur mbH, Hafentörn 3, 25761, Büsum, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schade |D Rüdiger |u Institute of Virology, Charité-Universitätsmedizin Berlin, 10117, Berlin, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Schulz |D Carsten |u Gesellschaft für Marine Aquakultur mbH, Hafentörn 3, 25761, Büsum, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wuertz |D Sven |u Department of Aquaculture and Ecophysiology, Leibnitz Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587, Berlin, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Aquaculture International |d Springer International Publishing |g 23/2(2015-04-01), 427-438 |x 0967-6120 |q 23:2<427 |1 2015 |2 23 |o 10499 | ||