caa a22 4500 605464979 CHVBK 20210128100300.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10499-014-9830-2 doi (NATIONALLICENCE)springer-10.1007/s10499-014-9830-2 Parentage determination in golden mandarin fish ( Siniperca scherzeri ) based on microsatellite DNA markers [Elektronische Daten] [Min Yang, Wenjie Guo, Xu-Fang Liang, Changxu Tian, Liyuan Lv, Kecheng Zhu, Hanping Wang] Retaining pedigree information and genetic diversity in each generation is essential but difficulty in selective breeding of aquaculture species. In this study, simulations and real data analysis were performed to examine the power of microsatellite markers in parentage determination of golden mandarin fish (Siniperca scherzeri). Simulations based on allele frequency data from the population of golden mandarin fish showed that the power of six loci to exclude false parents was higher than 95% and that of seven loci over 97%. When marker data from seven loci were combined, the accuracy of assignment to one true parental pair was up to 91.6% with known parental and filial information. The marker panel tested in mixed families, 89.5% of progeny was correctly assigned to their parental pairs. The mismatches caused by scoring errors at microsatellites loci were the major reason for the discrepancies between simulations and real data analysis. Taking these results into considerations, it was concluded that microsatellite markers can be used as a tool, alternative to physical tagging, to maintain pedigree information in selection programs of golden mandarin fish. Springer International Publishing Switzerland, 2014 Aquaculture nationallicence Golden mandarin fish nationallicence Microsatellite marker nationallicence Parentage determination nationallicence Selective breeding program nationallicence Yang Min College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut Guo Wenjie College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut Liang Xu-Fang College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut Tian Changxu College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut Lv Liyuan College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut Zhu Kecheng College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut Wang Hanping Aquaculture Genetics and Breeding Laboratory, Ohio State University South Centers, 1864 Shyville Road, 45661, Piketon, OH, USA aut Aquaculture International Springer International Publishing 23/2(2015-04-01), 499-507 0967-6120 23:2<499 2015 23 10499 https://doi.org/10.1007/s10499-014-9830-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9830-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Min College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Guo Wenjie College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Liang Xu-Fang College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Tian Changxu College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Lv Liyuan College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhu Kecheng College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Wang Hanping Aquaculture Genetics and Breeding Laboratory, Ohio State University South Centers, 1864 Shyville Road, 45661, Piketon, OH, USA aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/2(2015-04-01), 499-507 0967-6120 23:2<499 2015 23 10499