caa a22 4500 605464995 CHVBK 20210128100300.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10499-014-9827-x doi (NATIONALLICENCE)springer-10.1007/s10499-014-9827-x How many strains are required to set an epidemiological cut-off value for MIC values determined for bacteria isolated from aquatic animals? [Elektronische Daten] [Peter Smith, Göran Kronvall] Normalised resistance interpretation was applied to the estimation of epidemiological cut-off values from data sets that contained >150 observations from fully susceptible wild-type (WT) strains for six bacterial groups/antibiotic combinations. A series of 20 subsets containing 10, 20, 30 and 40 WT observations were randomly generated from these six larger data sets. The epidemiological cut-off values estimated using these 480 small data sets were compared with those estimated from their larger, parental data sets. Eighty-seven per cent of the 360 epidemiological cut-off values estimated from subsets that contained ≥20 WT observations were in agreement with those estimated from their respective larger sets. The remaining 13% differed only by one doubling dilution. These calculations suggest that small strain sets can be used to set epidemiological cut-off values for MIC data without unacceptable loss of precision. It is concluded that the design of experiments intended to generate the data necessary to set epidemiological cut-off values should aim to include observations on at least 30 WT observations. Springer International Publishing Switzerland, 2014 Antibiotic susceptibility nationallicence Epidemiological cut-off values nationallicence MIC nationallicence CLSI test protocols nationallicence M49-A nationallicence Precision nationallicence Sample size nationallicence Smith Peter Department of Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland aut Kronvall Göran Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden aut Aquaculture International Springer International Publishing 23/2(2015-04-01), 465-470 0967-6120 23:2<465 2015 23 10499 https://doi.org/10.1007/s10499-014-9827-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10499-014-9827-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith Peter Department of Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland aut NATIONALLICENCE 700 1- Kronvall Göran Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden aut NATIONALLICENCE 773 0- Aquaculture International Springer International Publishing 23/2(2015-04-01), 465-470 0967-6120 23:2<465 2015 23 10499