caa a22 4500 605465649 CHVBK 20210128100303.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00769-015-1168-6 doi (NATIONALLICENCE)springer-10.1007/s00769-015-1168-6 Comparison of exact matching and calibration curve quantification methods for glucose in human serum GC-IDMS analysis and their application for the development of certified reference materials [Elektronische Daten] [Juliana Barrabin, Bruno Garrido, Gabriella Machado, Wagner Wollinger, Rodrigo Freitas, Ana Feliciano, Marcia de la Cruz] The establishment of a precise and accurate method for the determination of the analyte is the first step in the development of certified reference materials (CRMs), which play a key role in metrological traceability. To assist the production of a glucose CRM in human serum for clinical chemistry routine tests, we developed a gas chromatography-isotope dilution mass spectrometry method using trimethylsilylation and performed two calibration techniques: exact matching approach and calibration curve. Performance of the method and calibration techniques were evaluated for their suitability for the characterization of a CRM candidate carrying out studies to assign the property value and to assure homogeneity as well as short- and long-term stability. Both calibration approaches were equivalent in terms of accuracy and precision and presented bias lower than 1.0% with relative standard deviations lower than 3.0%. Relative measurement uncertainties obtained with the exact matching approach calibration method were between 1.0 % and 2.5% and were lower than the ones obtained with calibration curve method. This finding justifies the use of the time-consuming exact matching method for the assignment of the property value, while the calibration curve procedure suits the other certification studies. Springer-Verlag Berlin Heidelberg, 2015 Exact matching approach nationallicence Uncertainty of measurement nationallicence GC-IDMS nationallicence Glucose analysis nationallicence Certified reference material nationallicence Barrabin Juliana National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut Garrido Bruno National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut Machado Gabriella National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut Wollinger Wagner National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut Freitas Rodrigo National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut Feliciano Ana National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut de la Cruz Marcia Institute of Chemistry, Federal University of Rio de Janeiro - UFRJ, 21941-909, Rio de Janeiro, RJ, Brazil aut Accreditation and Quality Assurance Springer Berlin Heidelberg 20/6(2015-12-01), 483-493 0949-1775 20:6<483 2015 20 769 https://doi.org/10.1007/s00769-015-1168-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00769-015-1168-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barrabin Juliana National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut NATIONALLICENCE 700 1- Garrido Bruno National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut NATIONALLICENCE 700 1- Machado Gabriella National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut NATIONALLICENCE 700 1- Wollinger Wagner National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut NATIONALLICENCE 700 1- Freitas Rodrigo National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut NATIONALLICENCE 700 1- Feliciano Ana National Institute of Metrology, Quality and Technology - Inmetro, Av Nossa Senhora das Graças, 50, 25250-020, Duque de Caxias, RJ, Brazil aut NATIONALLICENCE 700 1- de la Cruz Marcia Institute of Chemistry, Federal University of Rio de Janeiro - UFRJ, 21941-909, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 773 0- Accreditation and Quality Assurance Springer Berlin Heidelberg 20/6(2015-12-01), 483-493 0949-1775 20:6<483 2015 20 769