caa a22 4500 605465835 CHVBK 20210128100304.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00769-015-1129-0 doi (NATIONALLICENCE)springer-10.1007/s00769-015-1129-0 Proposed statistical analysis to evaluate qualitative proficiency testing of Salmonella serotyping [Elektronische Daten] [Marzia Mancin, Lisa Barco, Cristina Saccardin, Antonia Ricci] Salmonella is one of the main causes of foodborne illnesses in humans worldwide. More than 2500 different serotypes have been described so far. Correct identification of the serotype is essential, since the identification of different serotypes in animal populations and foodstuffs can lead to the application of different corrective measures according to current European food and animal legislation. Since 2007, Istituto Zooprofilattico Sperimentale delle Venezie, in its capacity as Italian National Reference Laboratory for Salmonella, has organised one proficiency test (PT) for Salmonella serotyping per year. The performance of the participant laboratories is assessed based on their capability to correctly identify serotypes of 20 selected Salmonella strains. To evaluate the overall PT performance, a detailed descriptive analysis of all results (identification of serotype by somatic and flagellar antigens for each strain) has been performed. Moreover, the performance of each participant is assessed using the generalised Cohen's κ statistic for multiple categories. The aim of the present study is to describe the Salmonella serotyping PT, focusing on the statistical approach followed to analyse the data collected. As an example, analysis of data obtained during the last PT (2013) is reported. All participants (12 laboratories) obtained κ values indicative of substantial or almost perfect agreement with the expected results. The overall performance of the PT also indicated perfect agreement among participants (κ value equal to 0.8767). Moreover, from 2007 to 2013, the κ value over time was always significantly higher than 0.75, indicating a substantial agreement among all participants in the qualitative PT for Salmonella serotyping. Springer-Verlag Berlin Heidelberg, 2015 Proficiency testing nationallicence Salmonella spp nationallicence Serotyping nationallicence κ Statistic nationallicence Performance evaluation nationallicence Mancin Marzia Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut Barco Lisa Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut Saccardin Cristina Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut Ricci Antonia Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut Accreditation and Quality Assurance Springer Berlin Heidelberg 20/4(2015-08-01), 305-310 0949-1775 20:4<305 2015 20 769 https://doi.org/10.1007/s00769-015-1129-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00769-015-1129-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mancin Marzia Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut NATIONALLICENCE 700 1- Barco Lisa Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut NATIONALLICENCE 700 1- Saccardin Cristina Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut NATIONALLICENCE 700 1- Ricci Antonia Food Safety Department, Istituto Zooprofilattico Sperimentale delle Venezie, viale dell'Università 10, 35020, Legnaro, PD, Italy aut NATIONALLICENCE 773 0- Accreditation and Quality Assurance Springer Berlin Heidelberg 20/4(2015-08-01), 305-310 0949-1775 20:4<305 2015 20 769