caa a22 4500 605465878 CHVBK 20210128100304.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00769-015-1127-2 doi (NATIONALLICENCE)springer-10.1007/s00769-015-1127-2 PT as a tool to point out criticalities in the strategy for control of antibiotic residues in milk: the Italian experience [Elektronische Daten] [Anna Ferrini, Umberto Agrimi, Brunella Appicciafuoco, Renata Borroni, Gianni Ciccaglioni, Fabio Galati, Maria Massaro, Marina Patriarca] Screening represents the first step for antibiotic residues control; thus, screening methods play a key role in the entire control procedure. The Italian National Reference Laboratory (NRL) for veterinary antimicrobial residues in food from animal origin organized the first proficiency test (PT) for the screening of antibiotic residues in milk to assess the competence of the official control laboratories. The 38 participants received two series (respectively, at 1.8% and 3.5% of fat mass fraction) of eight blind lyophilized milk samples, spiked with cloxacillin (CLX), benzylpenicillin (PEN), sulfadiazine (SDZ) and oxytetracycline (OXY) at once and twice the respective maximum residue limit. In addition, an unspiked sample (blank) was also included in each series. Since the vast majority of false-negative results was associated with OXY, leading to low sensitivity and accuracy, laboratories performances were evaluated separately for OXY (sensitivity 16.44%, false-negative rate 62.25% and accuracy 44.54%) and, respectively, for CLX, PEN and SDZ assessed together (sensitivity 99.56%, false-negative rate 2.53% and accuracy 99.63%); the false-positive rate resulted equivalent to 0% and the specificity to 100%. The criticality identified by the PT was that OXY was not detectable under the routine screening analysis conditions adopted because of the use of only two screening methods (Delvotest SP-NT and CMT) both unfit for its detection at mass fractions of interest. As a consequence, the NRL provided guidance to participants to implement different and complementary screening methods. The effectiveness of the corrective actions undertaken by participants will be evaluated with the next PT. Springer-Verlag Berlin Heidelberg, 2015 Proficiency test nationallicence Screening methods nationallicence Milk nationallicence Antibiotic residues nationallicence Ferrini Anna Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Agrimi Umberto Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Appicciafuoco Brunella Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Borroni Renata Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Ciccaglioni Gianni Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Galati Fabio Information Technology Service, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Massaro Maria Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Patriarca Marina Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut Accreditation and Quality Assurance Springer Berlin Heidelberg 20/4(2015-08-01), 267-272 0949-1775 20:4<267 2015 20 769 https://doi.org/10.1007/s00769-015-1127-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00769-015-1127-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferrini Anna Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Agrimi Umberto Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Appicciafuoco Brunella Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Borroni Renata Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Ciccaglioni Gianni Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Galati Fabio Information Technology Service, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Massaro Maria Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 700 1- Patriarca Marina Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161, Rome, Italy aut NATIONALLICENCE 773 0- Accreditation and Quality Assurance Springer Berlin Heidelberg 20/4(2015-08-01), 267-272 0949-1775 20:4<267 2015 20 769