Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products
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| 024 | 7 | 0 | |a 10.1007/s00769-015-1108-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00769-015-1108-5 | ||
| 245 | 0 | 0 | |a Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products |h [Elektronische Daten] |c [Lutz Grohmann, Ralf Reiting, Dietrich Mäde, Steffen Uhlig, Kirsten Simon, Kirstin Frost, Gurinder Randhawa, Katrin Zur] |
| 520 | 3 | |a Presence of genetic modifications in rice products originating from China and imported to the European Union market is detected since 2006. Neither these products from China nor any other genetically modified rice lines are approved as food or feed in the EU. The transgenic rice varieties identified contain genetic elements and constructs coding for insect-resistance genes from Bacillus thuringiensis (Bt) coding for insecticidal crystal (cry) proteins. In particular, DNA sequences coding for codon-optimised or fused cry1Ab/c genes and constructs driven by the maize ubiquitin promoter (P-ubiZM1) were identified. For improved screening and identification of genetic modifications present in Asian rice products, two TaqMan-based real-time PCR assays targeting codon-optimised cry1Ab/Ac and the Pubi-cry construct have been developed. These assays have been validated in an international collaborative trial with 17 participants from 10 countries. Based on a new mathematical-statistical model and an adjusted experimental set-up of the collaborative trial, a close examination of the limit of detection (LOD 95%) and the probability of detection of the qualitative PCR assays was conducted. The evaluation of the method performance characteristics and results of the collaborative trial validation are presented. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Genetically modified organism (GMO) |2 nationallicence | |
| 690 | 7 | |a Cry1Ab |2 nationallicence | |
| 690 | 7 | |a Maize ubiquitin promoter (P-ubiZM1) |2 nationallicence | |
| 690 | 7 | |a Rice |2 nationallicence | |
| 690 | 7 | |a Detection method |2 nationallicence | |
| 690 | 7 | |a Real-time PCR |2 nationallicence | |
| 690 | 7 | |a Probability of detection (POD) |2 nationallicence | |
| 690 | 7 | |a Collaborative trial study |2 nationallicence | |
| 700 | 1 | |a Grohmann |D Lutz |u Federal Office of Consumer Protection and Food Safety, Mauerstr. 39-42, 10117, Berlin, Germany |4 aut | |
| 700 | 1 | |a Reiting |D Ralf |u Hessian State Laboratory, Druseltalstr.67, 34131, Kassel, Germany |4 aut | |
| 700 | 1 | |a Mäde |D Dietrich |u State Office for Consumer Protection, Freiimfelder Str. 68, 06112, Halle (Saale), Germany |4 aut | |
| 700 | 1 | |a Uhlig |D Steffen |u QuoData GmbH, Prellerstr. 14, 01309, Dresden, Germany |4 aut | |
| 700 | 1 | |a Simon |D Kirsten |u QuoData GmbH, Prellerstr. 14, 01309, Dresden, Germany |4 aut | |
| 700 | 1 | |a Frost |D Kirstin |u QuoData GmbH, Prellerstr. 14, 01309, Dresden, Germany |4 aut | |
| 700 | 1 | |a Randhawa |D Gurinder |u Genomic Resources Division, National Bureau of Plant Genetic Resources, Pusa Campus, 110012, New Delhi, India |4 aut | |
| 700 | 1 | |a Zur |D Katrin |u Federal Office of Consumer Protection and Food Safety, Mauerstr. 39-42, 10117, Berlin, Germany |4 aut | |
| 773 | 0 | |t Accreditation and Quality Assurance |d Springer Berlin Heidelberg |g 20/2(2015-04-01), 85-96 |x 0949-1775 |q 20:2<85 |1 2015 |2 20 |o 769 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00769-015-1108-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00769-015-1108-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grohmann |D Lutz |u Federal Office of Consumer Protection and Food Safety, Mauerstr. 39-42, 10117, Berlin, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Reiting |D Ralf |u Hessian State Laboratory, Druseltalstr.67, 34131, Kassel, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mäde |D Dietrich |u State Office for Consumer Protection, Freiimfelder Str. 68, 06112, Halle (Saale), Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Uhlig |D Steffen |u QuoData GmbH, Prellerstr. 14, 01309, Dresden, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Simon |D Kirsten |u QuoData GmbH, Prellerstr. 14, 01309, Dresden, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Frost |D Kirstin |u QuoData GmbH, Prellerstr. 14, 01309, Dresden, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Randhawa |D Gurinder |u Genomic Resources Division, National Bureau of Plant Genetic Resources, Pusa Campus, 110012, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zur |D Katrin |u Federal Office of Consumer Protection and Food Safety, Mauerstr. 39-42, 10117, Berlin, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Accreditation and Quality Assurance |d Springer Berlin Heidelberg |g 20/2(2015-04-01), 85-96 |x 0949-1775 |q 20:2<85 |1 2015 |2 20 |o 769 | ||