Development of Penicillin G biosensor based on Penicillinase enzymes immobilized ontobio-chips
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| 024 | 7 | 0 | |a 10.1007/s10544-014-9910-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-014-9910-0 | ||
| 245 | 0 | 0 | |a Development of Penicillin G biosensor based on Penicillinase enzymes immobilized ontobio-chips |h [Elektronische Daten] |c [Mohammed Rahman, Abdullah Asiri] |
| 520 | 3 | |a Penicillin G (Pen-G) biosensor was developed by immobilizing penicillinase enzymes (Pen-X) onto tiny bio-chips using thioglycolic acid self-assembled monolayer (TGA-SAM). The selective pen-G biosensor wasinvestigated by ferri/ferrocyanide couple using electrochemical method for catalytic hydrolysis of Pen-G/Pen-X in a very sensible approach. Pen-G was detected with modified bio-chip (Gold electrode of bio-chip/Thioglycolic acid/Penicillinase enzyme, in shortly AuE/TGA/Pen-X) by reliable cyclic voltammetric method at pH7.1 in room conditions. The AuE/TGA/Pen-X modified bio-chip sensor demonstrates good linearity (50.0nM to 5.0mM; R = 0.9987), low-detection limit (~1.26nM, SNR ~ 3), and higher sensitivity (~4.97μA.μM−1.cm−2), lowest-small sample volume (<70.0μL), good stability, selectivity, and reproducibility. To the best of our knowledge, this is the first statement in which such a very high sensitivity, low-detection limit, and low-sample volume are required for Pen-G biosensor using AuE/TGA/Pen-X bio-chips assembly. The results of these studies have introducedfor biomedical applications with interesting assembly (AuE/TGA/Pen-X) towards the development of selective Pen-G biosensors. The AuE/TGA-SAM system was implemented a facile approach to the integration of Pen-X/Pen-G fabricated bio-chips, which can offer analytical access to a large group of enzymes for wide range of biomedical applications in health-care fields. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Penicillin G |2 nationallicence | |
| 690 | 7 | |a TGA self-assembled monolayer |2 nationallicence | |
| 690 | 7 | |a Cyclic voltammetry |2 nationallicence | |
| 690 | 7 | |a Bio-chips |2 nationallicence | |
| 690 | 7 | |a Sensitivity |2 nationallicence | |
| 690 | 7 | |a Penicillinase |2 nationallicence | |
| 700 | 1 | |a Rahman |D Mohammed |u Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia |4 aut | |
| 700 | 1 | |a Asiri |D Abdullah |u Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-7 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-014-9910-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-014-9910-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rahman |D Mohammed |u Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Asiri |D Abdullah |u Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/1(2015-02-01), 1-7 |x 1387-2176 |q 17:1<1 |1 2015 |2 17 |o 10544 | ||