caa a22 4500 605479240 CHVBK 20210128100409.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-014-9910-0 doi (NATIONALLICENCE)springer-10.1007/s10544-014-9910-0 Development of Penicillin G biosensor based on Penicillinase enzymes immobilized ontobio-chips [Elektronische Daten] [Mohammed Rahman, Abdullah Asiri] Penicillin G (Pen-G) biosensor was developed by immobilizing penicillinase enzymes (Pen-X) onto tiny bio-chips using thioglycolic acid self-assembled monolayer (TGA-SAM). The selective pen-G biosensor wasinvestigated by ferri/ferrocyanide couple using electrochemical method for catalytic hydrolysis of Pen-G/Pen-X in a very sensible approach. Pen-G was detected with modified bio-chip (Gold electrode of bio-chip/Thioglycolic acid/Penicillinase enzyme, in shortly AuE/TGA/Pen-X) by reliable cyclic voltammetric method at pH7.1 in room conditions. The AuE/TGA/Pen-X modified bio-chip sensor demonstrates good linearity (50.0nM to 5.0mM; R = 0.9987), low-detection limit (~1.26nM, SNR ~ 3), and higher sensitivity (~4.97μA.μM−1.cm−2), lowest-small sample volume (<70.0μL), good stability, selectivity, and reproducibility. To the best of our knowledge, this is the first statement in which such a very high sensitivity, low-detection limit, and low-sample volume are required for Pen-G biosensor using AuE/TGA/Pen-X bio-chips assembly. The results of these studies have introducedfor biomedical applications with interesting assembly (AuE/TGA/Pen-X) towards the development of selective Pen-G biosensors. The AuE/TGA-SAM system was implemented a facile approach to the integration of Pen-X/Pen-G fabricated bio-chips, which can offer analytical access to a large group of enzymes for wide range of biomedical applications in health-care fields. Springer Science+Business Media New York, 2015 Penicillin G nationallicence TGA self-assembled monolayer nationallicence Cyclic voltammetry nationallicence Bio-chips nationallicence Sensitivity nationallicence Penicillinase nationallicence Rahman Mohammed Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia aut Asiri Abdullah Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-7 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-014-9910-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-014-9910-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rahman Mohammed Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia aut NATIONALLICENCE 700 1- Asiri Abdullah Center of Excellence for Advanced Materials Research (CEAMR) & Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, 21589, Jeddah, Saudi Arabia aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-7 1387-2176 17:1<1 2015 17 10544