caa a22 4500 605479259 CHVBK 20210128100409.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10544-015-9936-y doi (NATIONALLICENCE)springer-10.1007/s10544-015-9936-y A reusable device for electrochemical applications of hydrogel supported black lipid membranes [Elektronische Daten] [Agnieszka Mech-Dorosz, Arto Heiskanen, Sania Bäckström, Mark Perry, Haseena Muhammad, Claus Hélix-Nielsen, Jenny Emnéus] Black lipid membranes (BLMs) are significant in studies of membrane transport, incorporated proteins/ion transporters, and hence in construction of biosensor devices. Although BLMs provide an accepted mimic of cellular membranes, they are inherently fragile. Techniques are developed to stabilize them, such as hydrogel supports. In this paper, we present a reusable device for studies on hydrogel supported (hs) BLMs. These are formed across an ethylene tetrafluoroethylene (ETFE) aperture array supported by the hydrogel, which is during in situ polymerization covalently "sandwiched” between the ETFE substrate and a gold electrode microchip, thus allowing direct electrochemical studies with the integrated working electrodes. Using electrochemical impedance spectroscopy (EIS), X-ray photoelectron spectroscopy and contact angle measurements, we demonstrate the optimized chemical modifications of the gold electrode microchips and plasma modification of the ETFE aperture arrays facilitating covalent "sandwiching” of the hydrogel. Both fluorescence microscopy and EIS were used to demonstrate the induced spontaneous thinning of a deposited lipid solution, leading to formation of stabilized hsBLMs on average in 10min. The determined specific membrane capacitance and resistance were shown to vary in the range 0.31-0.49μF/cm2 and 45-65 kΩ cm2, respectively, corresponding to partially solvent containing BLMs with an average life time of 60-80min. The characterized hsBLM formation and devised equivalent circuit models lead to a schematic model to illustrate lipid molecule distribution in hydrogel-supported apertures. The functionality of stabilized hsBLMs and detection sensitivity of the platform were verified by monitoring the effect of the ion transporter valinomycin. Springer Science+Business Media New York, 2015 Hydrogel supported black lipid membrane nationallicence Covalent tethering of hydrogel nationallicence ETFE aperture array nationallicence Plasma modification of ETFE nationallicence Spontaneous thinning of lipid layer nationallicence Impedance spectroscopy nationallicence Mech-Dorosz Agnieszka Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut Heiskanen Arto Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut Bäckström Sania Aquaporin A/S, Ole Maaløes Vej 3, 2200, Copenhagen N, Denmark aut Perry Mark Aquaporin A/S, Ole Maaløes Vej 3, 2200, Copenhagen N, Denmark aut Muhammad Haseena Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut Hélix-Nielsen Claus Aquaporin A/S, Ole Maaløes Vej 3, 2200, Copenhagen N, Denmark aut Emnéus Jenny Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-13 1387-2176 17:1<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9936-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9936-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mech-Dorosz Agnieszka Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Heiskanen Arto Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Bäckström Sania Aquaporin A/S, Ole Maaløes Vej 3, 2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Perry Mark Aquaporin A/S, Ole Maaløes Vej 3, 2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Muhammad Haseena Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 700 1- Hélix-Nielsen Claus Aquaporin A/S, Ole Maaløes Vej 3, 2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Emnéus Jenny Department of Micro- and Nanotechnology, Technical University of Denmark, Produktionstorvet 423, 2800, Kgs. Lyngby, Denmark aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/1(2015-02-01), 1-13 1387-2176 17:1<1 2015 17 10544